Ellisen

Ellisen Lab

Research topics include: P53 and human tumorigenesis; breast cancer

Overview

Leif Ellisen, MD, PhD
Program Director
Center for Breast Cancer, Massachusetts General Hospital Cancer Center

Professor of Medicine
Harvard Medical School

Research Summary

Cancer therapy is being revolutionized through the development of more specific and less toxic treatment approaches that are collectively known as targeted therapeutics. A key to the successful application of targeted cancer therapy is the identification of specific genetic abnormalities within tumor cells that are not present in normal tissues. The Ellisen laboratory is broadly interested in identifying these genetic abnormalities, understanding how they influence the biology of cancer cells, and discovering how that biology can inform the selection of the most effective therapy for each patient. We address these questions through basic research studies of key tumor-cell signaling pathways and through molecular analysis of patient tumor samples conducted in partnership with collaborators in the fields of molecular diagnostics and computational biology. Our discoveries in the basic laboratory and through tumor analysis have led to clinical trials that seek to identify new predictive markers and new therapeutic strategies for breast and other cancers.

Read the Ellisen Lab's Annual Report in full 

 

Group Members

Leif W. Ellisen, MD, PhD
Principal Investigator

Group Members

  • Mihriban Karaayvaz, PhD
  • Siang Boon Koh, PhD
  • Karina Matissek, PhD
  • Aiko Nagayama, MD
  • Kristofer Patel, BA
  • Shuxi Qiao, PhD
  • Srinivas Vinod Saladi, PhD
  • Devika Salunke, MS
  • Sheng Sun, PhD

Research Projects

Our group is broadly interested in how  genetic abnormalities within cancer cells influence their biology and how that biology can, in turn, be exploited to therapeutic advantage. We address these questions through basic research studies of key tumor cell signaling pathways including p53, mTOR, and BRCA1/2. This work is complemented by molecular analysis of patient tumor samples conducted in partnership with collaborators in the fields of molecular diagnostics and computational biology. Our discoveries in the basic laboratory and through tumor analysis are being applied in ongoing clinical trials that seek to identify predictive markers for response to specific therapeutics for breast and other cancers. Our ability to work at the interface of basic tumor biology and therapeutic application is strongly supported by our network of collaborators and by the research and clinical infrastructure of the Mass General Cancer Center.

The p53 network in cancer biology and therapy

The p53 tumor suppressor is inactivated in more than 50% of sporadic human cancers, and patients carrying heterozygous germline p53 mutations show striking tumor predisposition. P53 encodes a transcription factor that functions as a key nodal point for integrating cellular responses to DNA damage. As such, p53 regulates genes involved in diverse cellular processes including cell cycle progression, apoptosis and angiogenesis. The identification of two p53-related genes, p63 and p73, provided a new paradigm in the study of p53. We and others have defined a functional network through which these factors interact in human tumorigenesis. These findings are likely to explain the observation that p63 is over-expressed in a broad variety of epithelial tumors, particularly squamous cell and breast carcinomas. Our recent work has revealed roles for p63 and p73 in a variety of cancers, while our success in defining novel functional interactions within the p53 family provides new therapeutic possibilities for treatment-refractory malignancies.

P53 and TOR-associated metabolic reprogramming in tumorigenesis

Our efforts to identify new pathways regulated by p53 family members have yielded surprising insights into the re-wiring of cellular metabolism that drives carcinogenesis. A central player in this effect is REDD1, a p53- regulated gene we identified that functions as a critical negative regulator of the mammalian Target of Rapamycin (mTOR) kinase. Most human tumors exhibit abnormalities of p53 and/or mTOR signaling, and our recent studies have demonstrated how REDD1 contributes to autophagy and metabolic homeostasis to suppress growth and metastatic dissemination. We are currently using animal models, in vitro studies, and biochemical approaches to understand key metabolic dependencies of tumors that can be exploited to therapeutic advantage.

BRCA1/2, hereditary cancer predisposition and triple-negative breast cancer

Germline mutations in the DNA repair genes BRCA1 and BRCA2 confer dramatically elevated risk of cancers of the breast, ovary, and pancreas, yet the precise pathogenesis of BRCA1/2-associated cancer remains to be elucidated. We have launched a systematic study of early events that give rise to these cancers through detailed molecular analysis of normal and pre-cancerous tissues from BRCA1/2 mutation carriers. Defining the altered signaling and early cooperating events in this context is likely to reveal new markers of breast cancer predispositionand new targets for prevention. This work is complemented by our detailed studies of triple-negative breast cancer (TNBC), an aggressive subtype that comprises 80% of tumors in BRCA1 mutation carriers. Our newly-launched Triple-Negative Breast Cancer Program integrates basic research, translational and clinical studies together with human tumor propagation and high-throughput drug screening, all focused on overcoming drug resistance and improving outcomes for patients with TNBC.

Research Positions

Postdoctoral Research Fellow (1)

One Postdoctoral Research Fellow position is immediately available in the Jackson 9 Laboratories to study the role of p53 family members in human tumorigenesis. The candidate must have recently received a PhD degree in the biological sciences, and be highly motivated and well versed in basic molecular biology and biochemical techniques. The Fellow will have simultaneous academic appointments at the Massachusetts General Hospital and Harvard Medical School. We are using a number of genetic and biochemical approaches, including analysis of gene expression profiling and mouse models, to characterize the cellular pathways activated by p53, the most commonly mutated gene in human cancer. Related to these studies is our work on the p53 family member p63, which plays important roles in both human tumorigenesis and human development. We have recently identified novel downstream targets of p53 and p63, characterization of which will give new insights and provide new diagnostic and therapeutic approaches to human cancer. The position provides a rich intellectual environment within a group of young investigators, with full integration into the large research communities of the Massachusetts General Hospital and Harvard.

Contact

Please email a brief cover letter and CV to:

Leif W. Ellisen, MD, PhD
Massachusetts General Hospital Cancer Center
GRJ-904
55 Fruit Street
Boston, MA 02114
Email: lellisen@partners.org

 

Publications

View a list of publications by researchers at the Ellisen Laboratory

Selected Publications

Saladi, SV, Ross K, Karaayvaz M, Tata PR, Mou H, Rajagopal J, Ramaswamy S, and Ellisen LW. ACTL6A is co-Amplified with p63 in Squamous Cell Carcinoma to Drive YAP Activation, Regenerative Proliferation and Poor Prognosis. Cancer Cell 2017 31:35-49.

Qiao S, Dennis M, Song X, Vadysirisack DD, Salunke D, Nash Z, Yang Z, Liesa M, Yoshioka J, Matsuzawa S, Shirihai OS, Lee RT, Reed JC, Ellisen LW. A REDD1/TXNIP pro-oxidant complex regulates ATG4B activity to control stress-induced autophagy and sustain exercise capacity. Nature Communications. 2015 Apr 28;6:7014.

Isakoff SJ, Mayer EL, He L, Traina TA, Carey LA, Krag K, Rugo H, Liu MC, Stearns V, Come SE, Timms K, Hartman A-R, Borger DR, Finkelstein DM, Garber JE, Ryan PE , Winer EP, Goss PE, Ellisen LW. TBCRC009: A multi-center Phase II clinical trial of platinum monotherapy with biomarker assessment in metastatic triple-negative breast cancer. J Clin Oncol 2015 33:1902-9.

Forster N, Saladi SV, Van Bragt M, Sfondouris ME, Jones FE, Li Z, and Ellisen LW. Basal cell signaling by p63 controls luminal progenitor function and lactation via NRG1. Developmental Cell 2014; 28:147-60.

Ramsey M, Wilson C, Ory B, Rothenberg SM, Faquin W, Mills AA, Ellisen LW. FGFR2 Signaling Underlies p63 Oncogenic Function in Squamous Cell Carcinoma. J Clin Invest 2013; 123:3525-38.

He L, Torres-Lockhart K, Forster N, Ramkrishnan S, Greninger P, Garnett MJ, McDermott U, Rothenberg SM, Benes CH, and Ellisen LW. Mcl-1 and FBW7 control a dominant survival pathway underlying HDAC and Bcl-2 inhibitor synergy in squamous cell carcinoma. Cancer Discovery 2013; 3:324-37.

 

Contact

Contact Us

Ellisen Laboratory

GRJ-90455 Fruit Street Boston, MA 02114

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