CBRC CORES
1. Analytical and Quantitative Cytology Core Facility
2. Histology Core
3. Microscopy Core
4. Transgenic Drosophila Core
5. Transgenic Mouse Core
All CBRC Cores are available to non-CBRC investigators. Request forms and charge-back fee lists for services of all cores are available by contacting the core coordinators.
1. Analytical and Quantitative Cytology Core Facility
The Analytical and Quantitative Cytology Core Facility offers services and equipment to perform most flow cytometric assays.
The facility provides, but is not limited to, the following services: • Content and cell cycle analysis including the simultaneous measurement of multiple fluorescent probes;
• Immunofluorescence analysis (four-color analysis and maximal of eight-color analysis are routinely performed);
• Characterization of cell populations based on scattered light intensity measurement;
• Oxidative metabolism, Cytoplasmic calcium concentration, and pH measurement;
• Apoptosis assay and Viability assay (Membrane exclusion and metabolic viability);
• Bivariate and univariate chromosome analysis;
• Receptor-ligand interaction and Kinetic analysis;The facility also provides cell sorting including sorting of viable sterile population and spot blot. The sorting is performed with Mo-Flo, a high-speed sorter manufactured by Cytomation. It has the following unique features:
• A high-speed sorting runs at 25,000/Sec. with purity and recovery of > 90%;
• A 4-way sorting allows to sort for different populations simultaneously;
• A cyclone sorting is able to sort a signal cell into a 96-well plate for culture;
• A slide sorting can sort 1 to 4 different populates onto a slide directly.Contact Information
Core Director: Katia Georgopoulos, Ph.D.
Tel:617 726-4445
E-mail: katia.georgopoulos@cbrc2.mgh.harvard.eduCore Coordinator: Joanne Yetz-Aldape
Tel: 617 724-0247
E-mail: joanne.yetz-aldape@cbrc2.mgh.harvard.edu
2. Histology Core
The mission of the histology core is to provide routine histology tissue and slide preparation services to CBRC investigators, collaborators and affiliates.
Technical Services
The service include:
1-Paraffin tissue processing and sectioning.
2-Frozen tissue processing and sectioning.
3-Routine H&E staining.
4-Special histological stains.
5-Training on microtome and cryostat (This service is only available to CBRC investigators).Laboratory equipment:
Cryostat, Paraffin embedding station, Motorized rotary microtome, Stainer for routine histological stain and Tissue Processor.Contact Information
Core Director: Michael Detmar, M.D.
Tel: 617 724-1170
E-mail: michael.detmar@cbrc2.mgh.harvard.eduCore Coordinator: Myrtha Constant
Tel: 617 724-1169
E-mail: myrtha.constant@cbrc2.mgh.harvard.edu
3. Microscopy Core
The Confocal Microscopy Core is currently equipped with a Leica TCS NT 4D confocal microscope with the capability of collecting images simultaneously from three channels. Supervised by the microscopy core coordinator the microscope core has provided extensive imaging assistance for CBRC researchers and outside users. The core coordinator currently provides training in confocal and conventional microscopy and assistance in interpreting, analyzing and processing images by using image processing and graphics software. The core coordinator also serves as a source of information on confocal microscopy and immunohistochemistry and provides consultation for investigators whose research projects require these techniques. In addition to providing imaging assistance to the investigators in CBRC, the microscope core is also open to the MGH research community on a fee for service basis. As a new service, the coordinator can also generate semi-thin resin sections (>1um) using a recently acquired Leica microtome, on a fee-for-service basis.
Contact Information
Core Director: Kristin White, Ph.D.
Tel: 617 726-4440
E-mail: kristin.white@cbrc2.mgh.harvard.eduCore Coordinator: Hui Su, M.D.
Tel: 617 726-3145
E-mail: hui.su@cbrc2.mgh.harvard.edu
4. Transgenic Drosophila Core
The CBRC operates a core to make transgenic Drosophila strains. This service is provided by Doug Rennie, who is skilled in the injection technique used to introduce DNA into fly embryos. Mr. Rennie's services are available to any US lab on a "first-come, first serve", fee for service basis. The client provides the engineered plasmid DNA for P element-mediated transformation. Mr. Rennie makes reasonable efforts to ensure that the client obtains two independent transgenic strains. Currently, we are testing the feasibility of offering injection services for RNAi experiments. Please contact Dr. Laurel Raftery for further information about either service.
Please go here to link directly to the fly cores web site.Contact Information
Core Coordinator: Doug Rennie
Core Director: Laurel Raftery, Ph.D.
Tel: 617 726-1825
E-mail: laurel.raftery@cbrc2.mgh.harvard.edu
Tel: 617 726-2842
E-mail: doug.rennie@cbrc2.mgh.harvard.edu
5. Transgenic Mouse Core
The transgenic core provides services, which includes microinjection of oocytes and transplantation of embryos into foster recipients for the generation of transgenic mice. ES cells injections and blastocysts transplantation to generate knockout mice is also covered. Assistance is given to all investigators with their transgenic project related functions which include construct design, DNA preparation, mouse handling, genotyping, and colony generation. The facility is also equipped for the cryopreservation of 8-cell mouse embryos. Help is also provided to other transgenic facilities within and outside MGH with troubleshooting in problem areas.
Contact Information
Core Director: Katia Georgopoulos, Ph.D.
Tel:617 726-4445
E-mail: katia.georgopoulos@cbrc2.mgh.harvard.eduCore Coordinator: Lin Wu, Med.D., Ph.D.
Tel: 617 724-8124
E-mail: lin.wu@cbrc2.mgh.harvard.edu