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Summary
This Facility provides a monoclonal antibody production
service to research projects. Protein/peptides provided
by the individual investigators will be used to generate
mouse monoclonal antibodies.
Services
- Consultation to investigators in choosing appropriate
peptides or expressing
protein of interest as bacterial fusion protein.
- Ordering mice, injecting peptides/fusion proteins,
testing the mouse sera for positive response.
- Hybridoma fusion will be carried out on one or
more animals depending on response.
- Screening will be performed by ELISA and western
blot analyses.
- Systematic isolation of positive clones using the
Quixell Cell Transfer System, and
validation of hybridoma clones.
- Expansion of best clones (in consultation with
the investigators) and production of
concentrated supernatants
- If necessary we will arrange for ascites production.
Location
The facility is located at 185 Cambridge
Street, Simches Building, 5th floor
Director
and Staff
Director:
Vijaya Ramesh, Ph.D.
Phone: (617) 724-9733
Fax: (617) 726-3655
Email: ramesh@helix.mgh.harvard.edu
Team Leader: Nicole Smith
Phone: (617) 726-0841
Email: nsmith5@partners.org
Other Staff
Research Technician: Carolyn Polizzano
Phone: (617) 724-9559
Email: cpolizzano@partners.org
Fee
Schedule / Rates
Contact Nicole Smith at nsmith5@partners.org
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