Center for the Study of IBD

Morphology Core

Core Personnel:

Atul K. Bhan, MD
Phone: 617-726-2588
Dennis Brown, PhD
Phone: 617-726-5665
Yukako Yagi, PhD
Patricia Della Pelle
Research Technician

Mary McKee
Research Technician



Core Objectives:

The Morphology Core is designed to provide center investigators with the technical support, expertise and access to instrumentation that is necessary for their morphological and cell biological studies related to IBD. The use of these often sophisticated techniques to examine the localization of proteins important in the pathogenesis of various forms of bowel disease is essential to the work being performed by investigators involved in this center.

Services and Activities:

  • Core Methodology
  • Tissue and Cell Fixation 
  • Immunofluorescence Staining
    • Tyramide Activation
    • Immunoperoxidase Staining
  • Electron Microscopy
    • Immunogold Staining
    • Low Temperature Embedding in Lowicryl K4M and HM20
    • Freeze-substitution
    • Ultrathin Frozen Sections
  • Confocal Microscopy, Spinning Disk Confocal and TIRF Microscopy
    • Laser Scanning Confocal Microscopy
    • Spinning Disk Confocal Microscopy
    • Total Internal Reflection Fluorescence (TIRF) Microscopy
    • Calcium/pH ration Imaging
  • 3D and 4D in vitro and in vivo Analysis of Cell Interaction and Migration in the Mucosal Immune System
  • Digital Image Processing and Archiving
  • Central Antibody and Reagent Bank



The Morphology Core is based in two locations: the Program in Membrane Biology (PMB) in 8000 sq ft of space in the new Simches Research Center, MGH Histology Research Laboratory on the 8th floor of the Thier Research building and in the Immunopathology Unit equipped completely for light microscopy, immunofluorescence and immunoperoxidase. The Advanced Pathology Imaging Laboratory is located on the 2nd floor of the Jackson building. All the facilities are on the main campus of MGH and correspond to the locations of the laboratories of the Co-Directors.


Core Access:

The Morphology Core facilities have been made available to all members of the Center on as as-needed or by sign-up basis.