Confocal Microscope

Director: Pradeep Bhide, Ph.D.
Telephone: 617-726-5763
E-mail: bhide@helix.mgh.harvard.edu

Core Functions: This Core consists of a Zeiss LSM 5 Pascal laser confocal microscope. The Zeiss RGB vario laser module consists of argon laser (458/488/514 nm) and two helium-neon lasers (543 and 633 nm). A Zeiss Axiovert 200 fully motorized light microscope is available with fluorescence, bright-field, phase-contrast and Nomarski (DIC) capabilities. Image acquisition and analyses are performed using Zeiss LSM 5 Pascal Confocal Microscopy Software (Release 3.2) on 2 workstations. Zeiss Physiology software is available also. Live cell imaging is available using a Zeiss temperature controller with custom chamber and heating stage.

Examples of the specific services and analytical procedures available are:
• Double- and triple-labeling experiments
• High resolution images (2048 x 2048 pixels)
• Quantitative information for each channel
• 3-dimensional reconstruction
• Localized scans and FRAP (fluorescence recovery after photo-bleaching)
• Intracellular ion concentration
• Intracellular transport mechanism
• Dynamic events in live cells and tissues

Igor Bagayev – Technician
Email: Ibagayev@partners.org
Phone: 617-724-0588