In mammalian tissues water transport is of critical importance for osmotic balance and is facilitated by aquaporins (AQPs), which are integral membrane proteins forming aqueous pores.
Alfred N. Van Hoek, PhD
Instructor of Medicine
185 Cambridge Street, CPZN 8.208
Boston, MA 02114
Phone: (617) 724-8493
Fax: (617) 643-3182
In mammalian tissues, water transport is of critical importance for osmotic balance and is facilitated by aquaporins (AQPs), which are integral membrane proteins forming aqueous pores. The existence of 10 identified homologous aquaporins with diverse and complex tissue distributions, distinct biophysical, biochemical, physiological and morphological properties, appeals to novel insights of water and solute transport, which could lead to alternative treatments of hypertension, congestive heart failure, cirrhosis, the nephrotic syndrome, and other edema forming states. A detailed analysis of the molecular physiology of solvent/solute transport by functional domain exchange between water selective AQP4 channels and poly-selective AQP9 channels in combination with a scanning site-directed mutagenesis program is carried out. The production of active chimeras with mixed functional (selectivity for water and solute, sensitivity to phloretin and HgCl2) and structural (formation of orthogonal array aggregates or tetramers) properties are and will be studied in chimera-expressing oocytes. By osmotic volume flow and tracer studies water and solute transport is measured. Freeze fracture in combination with immunogold surface labeling is utilized to investigate morphology. A second focus is the purification of over-expressed AQP9 and mutant AQP9 proteins in baculovirus infected insect cells for the establishment of the molecular organization of AQP9 by 2-dimensional (2-D) crystallography. Antibodies raised against a synthetic AQP9-C-terminal peptide are utilized as a marker in SDS gels and Western blot analyses. The procedures for isolation include stripping and extraction by detergents, chromatographic maneuvers, and are similar to those used to purify AQP4. Because purified functional AQP4 is already available, low-resolution 2-D crystallography of AQP4 is expected and comprises a similar approach as has been done for AQP1. The prospect of detailed structural data of AQP4 and AQP9 is attractive and will lead to better understanding of pore structure and permeation selectivity, diversity, and distinct roles of aquaporins. It may also facilitate the design of water channel diuretics or "aquaretic" agents that could induce a diuresis in situations where present diuretics, such as furosemide, are ineffective.