Vector Development and Production
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Services
AAV
AAV vectors made available through the core carry AAV2 ITR elements serotyped with either AAV1, 2, 4, 5, 8, 9, or rh10 capsids. AAVs are purified by density gradient centrifugation, followed by FPLC chromatography. Featuring a number of fluorescent proteins, secreted and cytosolic luciferases, bicistronic constructs and tissue-specific promoters, our AAVs are useful for infecting cell cultures as well as tissues in living organisms.
|
ITEM |
PHS |
NON-PHS PRICE |
TITER |
|
AAV |
$1,500 |
$2,160 |
10 11- 10 13 gc/mL |
View a list of our AAV vectors
To view a guide to choosing the correctAAVserotype for your project, click here
To view a comparison of our product and prices to our competitors in the area, click here
Lentivirus
Lentiviral vectors made available through the Core are cary HIV-1 LTRs, pseudotyped with the VSV-G envelope protein.
|
ITEM |
PHS PRICE |
NON- PHS PRICE |
TITER |
|
Small-scale lentivirus |
$500 |
$720 |
10 6- 10 8 IU/mL |
|
Concentrated lentivirus |
$1,000 |
$1,440 |
10 9- 10 10 IU/mL |
To view a list of our lentiviral vectors, click here
To view a comparison of our product and prices to our competitors in the area, click here
Plasmid Prep
In order to achieve the highest viral titer possible, the MGH Vector Core requires sterile, endotoxin-free plasmid DNA from its customers. While commercial DNA prep kits are available, many labs find it more convenient and cost-effective to have us do this for them. Below is a table showing the cost for our Plasmid Preparation.
|
STARTING MATERIAL |
PHS PRICE |
NON-PHS PRICE |
|
LB-agar plate |
$175 |
$252 |
|
Glycerol stock |
$175 |
$252 |
|
Mini-prepped DNA |
$250 |
$360 |
|
Maxi-prepped DNA |
$250 |
$360 |
Plasmid Sharing
Coming soon!
Retrovirus
|
ITEM |
PHS PRICE |
NON-PHS PRICE |
TITER |
|
Concentrated retrovirus |
$1,200 |
$1,728 |
10 6- 10 8 IU/mL |
Virus Aliquots
|
VIRUS |
PHS PRICE |
NON-PHS PRICE |
|
AAV aliquot |
$250 |
$360 |
|
Lentivirus aliquot |
$150 |
$216 |
|
Concentrated lentivirus aliquot |
$200 |
$288 |