Cell Sorting at the Center for Regenerative Medicine

The flow cytometry laboratory is located at the Charles River Plaza location of the Center for Regenerative Medicine. Our facility has a customized BD FACSAria sorter, a BD LSRII analyzer,
two BD FACSCaliburs, and a Miltenyi AutoMACS magnetic cell sorter. We
also have multiple computers available for offline analysis.

Our BD FACSAria is a high-speed cell sorter (up to 25,000 cells/
second) and analyzer (up to 70,000 cells/second). Our custom built
machine includes five lasers (UV - 355 nm; Violet - 407 nm; Blue - 488
nm; Green - 532 nm; Red - 633 nm) and can view a variety of colors –
up to 17 fluorescence parameters. Sorting can be done into various
sized tubes (simultaneous collection of up to 4 populations), as well
as into plates (either single cell or multiple cells). All these
capabilities make it a highly flexible machine to meet the needs of
this growing research community.

Two BD FACSCaliburs are available for independent analysis. Each is
configured with two lasers (Blue - 488 nm and Red - 633 nm) and is
capable of up to four colors. Our Miltenyi autoMacs Separator is
available for high-speed magnetic cell sorting. Trained users may
access this machine at any time. We provide one-on-one hands-on
training for these machines, maintain them, and support self-run users
with any machine problems they may have.

We have two Apple iMacs for independent offline data analysis, with an
additional computer is available in the CRM computer room. We provide
hands-on training in FlowJo and ModFit LT for interested users. In
addition, you may book time with us to prepare data for publication.

Appointment booking is done via an online scheduler, which is
accessible from any computer 24 hours a day. The scheduler is
available at: http://harvardstemcellinstitute.org/CRM_Flow/

Contact Laura Prickett-Rice for more information.
(lprickett@partners.org).