Center for Regenerative Medicine

hESC Core Facility

The purpose of the human embryonic stem cell (hESC) Core Facility is to provide an institutional resource for hESC research at Massachusetts General Hospital.

The primary mission of the hESC Core Facility is to provide scientists from MGH and the Harvard Stem Cell Institute with the critical services to efficiently and successfully conduct human embryonic stem cell research. These services include:

1. Individual training and guidance in hESC culture, maintenance, transduction, differentiation and characterization.

2. Maintenance and availability of hESC lines with appropriate quality control.

3. Development of new protocols and tools to better facilitate the use of human embryonic stem cells.

4. Availability of a state-of-the-art cell culture room specifically designed for hESC research.

5. Providing an asylum for investigators interested in working with cell lines not listed in the NIH registry.

6. Providing a forum to discuss the bioethical issues surrounding this research in the form of a bi-monthly bioethics roundtable.

Dr. Laurence Daheron runs the Human Embryonic Stem Cell Core Facility at MGH. She encourages scientists at MGH and HSCI who are interested in hESC research to contact her at ldaheron@partners.org.

It is our hope that the availability of the Core Facility will promote further study of human embryonic stem cells and will help scientists achieve their scientific and medical goals.

The facility includes a modern cell culture room with four biological safety cell culture hoods (one exclusively used for virus preparation), six incubators (two of which are equipped to modulate the oxygen level), an inverted microscope and a stereoscopic zoom microscope.

The hESC lines used include: H1, H9 and HUES 1-17.

hESC Core Facility charges

MGH Center for Regenerative Medicine

Richard B. Simches Research Center
185 Cambridge Street
Boston, MA 02114

Phone: 617-724-5780
Fax: 617-724-2662
Email: lmtramontozzi@partners.org