Edward Ryan, MD

Ryan Laboratory

The Edward T. Ryan, MD Laboratory’s research focuses on host-bacterial pathogen interactions and immune responses, with a particular focus on enteric infections and the development of vaccines protective against enteric infections, and development of diagnostic assays.

About Us

Our research focuses on host-bacterial pathogen interactions and immune responses, with a particular focus on enteric infections and the development of vaccines protective against enteric infections, and development of diagnostic assays.  Specific focus areas include Vibrio cholerae, the cause of cholera, and Salmonella enterica, the causes of typhoid and paratyphoid fever.  We work in collaborative efforts with researchers at the International Centre for Diarrhoeal Disease Research in Dhaka Bangladesh (ICDDR,B).

Our laboratory is comprised of four post-doctoral fellows, three technicians, and two students.

Learn more about Edward T. Ryan, MD.

Our Team

Edward T. Ryan, MD

Ryan Lab Group
Ryan Laboratory Group Members

Edward T. Ryan, M.D. is the Director of Global Infectious Diseases at Massachusetts General Hospital, Professor of Medicine at Harvard Medical School, and Professor of Immunology and Infectious Diseases at Harvard School of Public Health. Dr. Ryan received his Bachelor’s degree in Biochemical Sciences from Princeton University, and a Doctorate in Medicine from Harvard University. He performed medical residency and fellowship training in infectious diseases at Mass General. Dr. Ryan received additional training in tropical medicine and infectious diseases at the London School of Hygiene & Tropical Medicine, was a Fellow in Human Rights & Medicine, Columbia University, and was an International Fellow, International Centre for Diarrhoeal Disease Research (ICDDR, B), Dhaka, Bangladesh.

Dr. Ryan's research focuses on clinical studies of illnesses associated with residing in, immigrating from, or traveling through resource-limited settings. His research is funded by the U.S. National Institute of Allergy and Infectious Diseases (NIAID) and the U.S. Centers for Disease Control & Prevention (CDC). Dr. Ryan is the principal investigator on research projects focusing on enteric vaccine development, host-pathogen studies on V. cholerae (the cause of cholera) and Salmonella enterica serovar Typhi (the cause of typhoid fever). Particular areas of focus include the application of high throughput genomic, proteomic, immunoproteomic, and web-based platform technologies to these illnesses. Dr. Ryan is also the Director of Global TravEpiNet, a CDC-supported national consortium focusing on global infectious diseases and evaluating vaccination strategies and use among global international travelers.  He is the principal investigator and program director for a Fogarty International Center, National Institutes of Health (NIH)-sponsored Training Program in Vaccine Development and Public Health between Harvard, Mass General and the ICDDR,B in Dhaka, Bangladesh. Dr. Ryan teaches tropical medicine and infectious diseases in the core curricula at Harvard Medical School, Harvard T.H. Chan School of Public Health and Harvard College, teaches students, residents and fellows at Mass General, and teaches in a number of post-graduate courses at Harvard Medical School and internationally.

Dr. Ryan has served on expert and advisory committees for the Institute of Medicine-National Academy of Sciences, U.S. Centers for Disease Control & Prevention, chaired the Clinical Research and Field Studies of Infectious Diseases Study section of the US NIH from 2006-2008, currently chairs the Standards and Treatment Guidelines Committee of the American Society of Tropical Medicine & Hygiene (ASTMH), and served as ASTMH President from 2009-2010. Dr. Ryan has been elected to Fellowship of the American College of Physicians, the Infectious Diseases Society of America, the American Society of Tropical Medicine & Hygiene, and the American Academy of Microbiology, and has published over 200 peer-reviewed journal articles and book chapters on enteric infections, infectious diseases, vaccines, and tropical medicine.  Dr. Ryan is a Senior Editor of Hunter's Tropical Medicine, 9th Edition.

Our Research

Immune responses during cholera

In a collaborative effort with researchers at the International Centre for Diarrhoeal Disease Research in Dhaka, Bangladesh (ICDDR,B), we are evaluating immune responses in humans infected with Vibrio cholerae, the cause of cholera. V. cholerae is endemic in over 50 countries, infects approximately 3-5 million individuals globally, and results in the death of approximately 100,000 individuals each year. Individuals most affected by cholera are those most impoverished, especially those lacking safe water and sanitary facilities, as well as individuals displaced by war, famine, disasters, and conflict. Cholera can be explosively epidemic, and the global burden of cholera may well increase with climate change, severe weather events, and increasing urbanization. V. cholerae is a human-restricted infection, and current cholera vaccines provide relatively short-term protection against disease. The mediators of protective immunity against cholera are poorly understood. To address this, we are applying a number of high throughput and platform technologies to assess innate and adaptive immune responses in humans with cholera and their household contacts, stratifying responses in the latter by subsequent protection from disease, and comparing responses in the former to those that occur in recipients of current cholera vaccines. The goal of these studies is to identify the mediators of protection against cholera, in order to advance improved prevention strategies, as well as to advance vaccine development and deployment.

Host-pathogen interactions during typhoid fever

In a collaborative effort with researchers at the International Centre for Diarrhoeal Disease Research in Dhaka, Bangladesh (ICDDR,B), we are evaluating host-pathogen interactions and immune responses in humans infected with Salmonella enterica serotype Typhi (the cause of typhoid fever) and S. Paratyphi (the cause of paratyphoid fever). Together, S. Typhi and S. Paratyphi cause approximately 20 million cases of enteric fever world-wide, resulting in approximately 200,000 deaths each year. Most of these deaths occur in impoverished children and young adults. Although usually caused by S. Typhi, one in four cases of enteric fever is caused by S. Paratyphi in many areas of the world. S. Typhi and S. Paratyphi are particularly common among urban residents in informal settlements and slums, although any individual lacking safe water and sanitary facilities is at risk of infection. Vaccines against S. Typhi provide only 50-60% protection against disease for 2-5 years, and, at present, there is no commercially available vaccine against S. Paratyphi. There is also no good point-of-care test to diagnose individuals with enteric fever, and S. Typhi and S. Paratyphi are becoming increasingly resistant to antimicrobial agents.To address this, we are evaluating host-pathogen interactions directly in humans infected with S. Typhi and S. Paratyphi. We are applying a number of technologies to evaluate innate and adaptive immune responses directly in humans infected with S. Typhi and S. Paratyphi in Bangladesh, and we are evaluating bacterial responses in humans during these human-restricted infections. The goal of these studies is to develop improved diagnostic assays, antimicrobial agents, and preventative strategies against these infections.

Immunization approaches for enteric infection

A fundamental challenge facing many enteric and mucosal vaccines is their inability to induce long-term protective immunity. Many of these vaccines may not be prominent inducers of memory cells, which play critical roles in mediating long-term protection against disease. Using our analysis of memory B and T cell induction during wild-type human enteric infection in Bangladesh, we are evaluating the ability of a number of vaccination approaches and strategies to induce long-term memory responses protective against mucosal and enteric infections, including development of enteric conjugate vaccines, and analysis of oral-transcutaneous prime-boosting approaches.

Global TravEpinet (GTEN)

To extend our analysis of infections associated with living in, traveling through, or immigrating from resource-limited areas, we also work with the U.S. Centers for Disease Control and Prevention through the GTEN (Global TravEpiNet; Global Travelers’ Epidemiology Network) Program. GTEN’s mission is to advance the health of American residents who travel internationally, as well as to lessen the likelihood of disease importation into home communities. GTEN-relate resources are available at Heading Home Healthy

Publications

References:

  1. The plasma and mucosal IgM, IgA, and IgG responses to the Vibrio cholerae O1 protein immunome in adults with cholera in Bangladesh.
    Charles RC, Nakajima R, Liang L, Jasinskas A, Berger A, Leung DT, Kelly M, Xu P, Kovác P, Giffen SR, Harbison JD, Chowdhury F, Khan AI, Calderwood SB, Bhuiyan TR, Harris JB, Felgner PL, Qadri F, Ryan ET. J Infect Dis. 2017 May 23. PMID: 28535267
  2. Missed Opportunities for Measles, Mumps, Rubella Vaccination Among Departing U.S. Adult Travelers Receiving Pretravel Health Consultations.
    Hyle EP, Rao SR, Jentes ES, Fiebelkorn AP, Hagmann SHF, Walker AT, Walensky RP, Ryan ET, LaRocque RC. Ann Intern Med. 2017 May 16. doi: 10.7326/M16-2249. PMID: 28505632
  3. Recommendations of the Advisory Committee on Immunization Practices for Use of Cholera Vaccine.
    Wong KK, Burdette E, Mahon BE, Mintz ED, Ryan ET, Reingold AL. MMWR Morb Mortal Wkly Rep. 2017 May 12;66(18):482-485. doi: 10.15585/mmwr.mm6618a6. PMID: 28493859
  4. Conjugate Vaccines from Bacterial Antigens by Squaric Acid Chemistry: A Closer Look.
    Xu P, Kelly M, Vann WF, Qadri F, Ryan ET, Kováč P. Chembiochem. 2017 Apr 18;18(8):799-815. doi: 10.1002/cbic.201600699. Epub 2017 Mar 23. PMID: 28182850
  5. Preparing Pediatric International Travelers for Travelers' Diarrhea: Insights From the Global TravEpiNet.
    Hagmann SH, LaRocque RC, Ryan ET. Pediatr Infect Dis J. 2017 Feb;36(2):242-243. doi: 10.1097/INF.0000000000001402. PMID: 28079839
  6. Single-Cell Analysis of the Plasmablast Response to Vibrio cholerae Demonstrates Expansion of Cross-Reactive Memory B Cells.
    Kauffman RC, Bhuiyan TR, Nakajima R, Mayo-Smith LM, Rashu R, Hoq MR, Chowdhury F, Khan AI, Rahman A, Bhaumik SK, Harris L, O'Neal JT, Trost JF, Alam NH, Jasinskas A, Dotsey E, Kelly M, Charles RC, Xu P, Kováč P, Calderwood SB, Ryan ET, Felgner PL, Qadri F, Wrammert J, Harris JB. MBio. 2016 Dec 20;7(6). pii: e02021-16. doi: 10.1128/mBio.02021-16. PMID: 27999163
  7. Characteristics of US Travelers to Zika Virus-Affected Countries in the Americas, March 2015-October 2016.
    Lammert S, Walker AT, Erskine S, Rao SR, Esposito DH, Ryan ET, Robbins GK, LaRocque RC. Emerg Infect Dis. 2017 Feb;23(2):324-327. doi: 10.3201/eid2302.161292. PMID: 27926355
  8. Biomarkers of Environmental Enteropathy are Positively Associated with Immune Responses to an Oral Cholera Vaccine in Bangladeshi Children.
    Uddin MI, Islam S, Nishat NS, Hossain M, Rafique TA, Rashu R, Hoq MR, Zhang Y, Saha A, Harris JB, Calderwood SB, Bhuiyan TR, Ryan ET, Leung DT, Qadri F. PLoS Negl Trop Dis. 2016 Nov 8;10(11):e0005039. doi: 10.1371/journal.pntd.0005039. PMID: 27824883
  9. Refusal of recommended travel-related vaccines among U.S. international travellers in Global TravEpiNet.
    Lammert SM, Rao SR, Jentes ES, Fairley JK, Erskine S, Walker AT, Hagmann SH, Sotir MJ, Ryan ET, LaRocque RC. J Travel Med. 2016 Oct 30;24(1). pii: taw075. PMID: 27799502
  10. Immune Responses to an Oral Cholera Vaccine in Internally Displaced Persons in South Sudan.
    Iyer AS, Bouhenia M, Rumunu J, Abubakar A, Gruninger RJ, Pita J, Lino RL, Deng LL, Wamala JF, Ryan ET, Martin S, Legros D, Lessler J, Sack DA, Luquero FJ, Leung DT, Azman AS. Sci Rep. 2016 Oct 24;6:35742. doi: 10.1038/srep35742. PMID: 27775046
  11. Personal Actions to Minimize Mosquito-Borne Illnesses, Including Zika Virus.
    LaRocque RL, Ryan ET. Ann Intern Med. 2016 Oct 18;165(8):589-590. doi: 10.7326/M16-1397. PMID: 27399646
  12. Medical Considerations before International Travel.
    LaRocque RC, Ryan ET. N Engl J Med. 2016 Oct 13;375(15):e32. PMID: 27732815
  13. A magneto-DNA nanoparticle system for the rapid and sensitive diagnosis of enteric fever.
    Park KS, Chung HJ, Khanam F, Lee H, Rashu R, Bhuiyan MT, Berger A, Harris JB, Calderwood SB, Ryan ET, Qadri F, Weissleder R, Charles RC. Sci Rep. 2016 Sep 8;6:32878. doi: 10.1038/srep32878. PMID: 27605393
  14. Antibody Secreting Cell Responses following Vaccination with Bivalent Oral Cholera Vaccine among Haitian Adults.
    Matias WR, Falkard B, Charles RC, Mayo-Smith LM, Teng JE, Xu P, Kováč P, Ryan ET, Qadri F, Franke MF, Ivers LC, Harris JB. PLoS Negl Trop Dis. 2016 Jun 16;10(6):e0004753. doi: 10.1371/journal.pntd.0004753. PMID: 27308825
  15. Erratum: Chemoproteomic profiling of host and pathogen enzymes active in cholera.
    Hatzios SK, Abel S, Martell J, Hubbard T, Sasabe J, Munera D, Clark L, Bachovchin DA, Qadri F, Ryan ET, Davis BM, Weerapana E, Waldor MK. Nat Chem Biol. 2016 May 18;12(6):466. doi: 10.1038/nchembio0616-466. PMID: 27191648
  16. Comparison of the Performance of the TPTest, Tubex, Typhidot and Widal Immunodiagnostic Assays and Blood Cultures in Detecting Patients with Typhoid Fever in Bangladesh, Including Using a Bayesian Latent Class Modeling Approach.
    Islam K, Sayeed MA, Hossen E, Khanam F, Charles RC, Andrews J, Ryan ET, Qadri F. PLoS Negl Trop Dis. 2016 Apr 8;10(4):e0004558. doi: 10.1371/journal.pntd.0004558. PMID: 27058877
  17. O-specific polysaccharide (OSP)-specific memory B cell responses in young children, older children and adults infected with Vibrio cholerae O1 Ogawa in Bangladesh. Aktar A, Rahman MA, Afrin S, Faruk MO, Uddin T, Akter A, Sami MI, Yasmin T, Chowdhury F, Khan AI, Leung DT, LaRocque RC, Charles RC, Bhuiyan TR, Mandlik A, Bufano MK, Kováč P, Xu P, Calderwood SB, Harris JB, Qadri F, Ryan ET.  O-specific polysaccharide (OSP)-specific memory B cell responses in young children, older children and adults infected with Vibrio cholerae O1 Ogawa in Bangladesh. Clin Vaccine Immunol (in press). PMC in process.
  18. Comparison of the Performance of the TPTest, Tubex, Typhidot and Widal Immunodiagnostic Assays and Blood Cultures in Detecting Patients with Typhoid Fever in Bangladesh, Including Using a Bayesian Latent Class Modeling Approach. Islam K, Sayeed MA, Hossen E, Khanam F, Charles RC, Andrews J, Ryan ET, Qadri F.  Comparison of the performance of the TPTest, Tubex, Typhidot and Widal immunodiagnostic assays and blood cultures in detecting patients with typhoid fever in Bangladesh, including using a Bayesian latent class modeling approach.  PLoS Negl Trop Dis. 2016 Apr 8;10(4):e0004558.  PMC4825986
  19. Development of a simple, peripheral blood-based lateral-flow dipstick assay for accurate detection of patients with enteric fever. Khan IH, Sayeed MA, Sultana N, Islam K, Amin J, Faruk MO, Khan U, Khanam F, Ryan ET, Qadri F. Development of a simple, peripheral blood-based lateral-flow dipstick assay for accurate detection of patients with enteric fever. Clin Vaccine Immunol (in press). PMC in process.
  20. Chemoproteomic profiling of host and pathogen enzymes active in cholera Hatzios SK, Abel S, Martell J, Hubbard T, Sasabe J, Munera D, Clark L, Bachovchin DA, Qadri F, Ryan ET, Davis BM, Weerapana E, Waldor MK.  Chemoproteomic profiling of host and pathogen enzymes active in cholera.  Nature Chemical Biology. 2016 Apr;12(4):268-74. PMC4765928.
  21. Safe and Healthy International Travel. Lammert S, Ryan ET, LaRoque RC. JAMA. 2015 Dec 15;314(23):2580. doi: 10.1001/jama.2015.15012. PMID: 26670985
  22. Vibrio cholerae Serogroup O139: Isolation from Cholera Patients and Asymptomatic Household Family Members in Bangladesh between 2013 and 2014. Chowdhury F, Mather AE, Begum YA, Asaduzzaman M, Baby N, Sharmin S, Biswas R, Ikhtear Uddin M, LaRocque RC, Harris JB, Calderwood SB, Ryan ET, Clemens JD, Thomson NR, Qadri F. PLoS Negl Trop Dis. 2015 Nov 12;9(11):e0004183. doi: 10.1371/journal.pntd.0004183. eCollection 2015 Nov.
    PMID: 26562418 Free PMC Article
  23. Enumeration of gut-homing β7-positive, pathogen-specific antibody secreting cells in whole blood in enterotoxigenic Escherichia coli and Vibrio cholerae-infected patients using an ELISPOT technique. Bhuiyan TR, Hoq MR, Nishat NS, Al Mahbuba D, Rashu R, Islam K, Hossain L, Dey A, Harris JB, Ryan ET, Calderwood SB, Svennerholm AM, Qadri F. Clin Vaccine Immunol. 2015 Oct 28. pii: CVI.00526-15. [Epub ahead of print] PMID: 26512047
  24. Pre-Travel Medical Preparation of Business and Occupational Travelers: An Analysis of the Global TravEpiNet Consortium, 2009 to 2012. Khan NM, Jentes ES, Brown C, Han P, Rao SR, Kozarsky P, Hagmann SH, LaRocque RC, Ryan ET; Global TravEpiNet Consortium (GTEN). J Occup Environ Med. 2015 Oct 15. [Epub ahead of print] PMID: 26479857
  25. Fluorescence Polarization Based Nucleic Acid Testing for Rapid and Cost-Effective Diagnosis of Infectious Disease. Park KS, Charles RC, Ryan ET, Weissleder R, Lee H. Chemistry. 2015 Nov 9;21(46):16359-63. doi: 10.1002/chem.201502934. Epub 2015 Sep 30. PMID: 26420633
  26. The increased severity in patients presenting to hospital with diarrhea in Dhaka, Bangladesh since the emergence of the hybrid strain of Vibrio cholerae O1 is not unique to cholera patients. Chowdhury F, Kuchta A, Khan AI, Faruque AS, Calderwood SB, Ryan ET, Qadri F. Int J Infect Dis. 2015 Nov;40:9-14. doi: 10.1016/j.ijid.2015.09.007. Epub 2015 Sep 25. PMID: 26409202 Free Article
  27. Factors Associated with Non-typhoidal Salmonella Bacteremia versus Typhoidal Salmonella Bacteremia in Patients Presenting for Care in an Urban Diarrheal Disease Hospital in Bangladesh. Shahunja KM, Leung DT, Ahmed T, Bardhan PK, Ahmed D, Qadri F, Ryan ET, Chisti MJ. PLoS Negl Trop Dis. 2015 Sep 11;9(9):e0004066. doi: 10.1371/journal.pntd.0004066. eCollection 2015 Sep.
    PMID: 26361076 Free PMC Article
  28. Immunocompromised Travelers: Demographic Characteristics, Travel Destinations, and Pretravel Health Care from the U.S. Global TravEpiNet Consortium. Schwartz BS, Rosen J, Han PV, Hynes NA, Hagmann SH, Rao SR, Jentes ES, Ryan ET, LaRocque RC; Global TravEpiNet Consortium. Am J Trop Med Hyg. 2015 Nov 4;93(5):1110-6. doi: 10.4269/ajtmh.15-0185. Epub 2015 Aug 24. PMID: 26304922
  29. Cholera in pregnancy: Clinical and immunological aspects. Khan AI, Chowdhury F, Leung DT, Larocque RC, Harris JB, Ryan ET, Calderwood SB, Qadri F.
    Int J Infect Dis. 2015 Oct;39:20-4. doi: 10.1016/j.ijid.2015.08.006. Epub 2015 Aug 14.
  30. A Cholera Conjugate Vaccine Containing O-specific Polysaccharide (OSP) of V. cholerae O1 Inaba and Recombinant Fragment of Tetanus Toxin Heavy Chain (OSP:rTTHc) Induces Serum, Memory and Lamina Proprial Responses against OSP and Is Protective in Mice. Sayeed MA, Bufano MK, Xu P, Eckhoff G, Charles RC, Alam MM, Sultana T, Rashu MR, Berger A, Gonzalez-Escobedo G, Mandlik A, Bhuiyan TR, Leung DT, LaRocque RC, Harris JB, Calderwood SB, Qadri F, Vann WF, Kováč P, Ryan ET. PLoS Negl Trop Dis. 2015 Jul 8;9(7):e0003881. doi: 10.1371/journal.pntd.0003881. eCollection 2015 Jul. PMID: 26154421
  31. Concurrent Pneumonia in Children Under 5 Years of Age Presenting to a Diarrheal Hospital in Dhaka, Bangladesh. Leung DT, Das SK, Malek MA, Qadri F, Faruque AS, Chisti MJ, Ryan ET. Am J Trop Med Hyg. 2015 Jul 6. pii: 15-0074. PMID: 26149863
  32. Plasma Leptin Levels in Children Hospitalized with Cholera in Bangladesh. Falkard B, Uddin T, Arifur Rahman M, Franke MF, Aktar A, Uddin MI, Bhuiyan TR, Leung DT, Charles RC, Larocque RC, Harris JB, Calderwood SB, Qadri F, Ryan ET. Am J Trop Med Hyg. 2015 Jun 8. pii: 15-0172. PMID: 26055740
  33. Gut microbial succession follows acute secretory diarrhea in humans. David LA, Weil A, Ryan ET, Calderwood SB, Harris JB, Chowdhury F, Begum Y, Qadri F, LaRocque RC, Turnbaugh PJ. MBio. 2015 May 19;6(3):e00381-15. doi: 10.1128/mBio.00381-15. PMID: 25991682
  34. Diagnostics for invasive Salmonella infections: Current challenges and future  directions. Andrews JR, Ryan ET. Vaccine. 2015 Jun 19;33 Suppl 3:C8-C15. doi: 10.1016/j.vaccine.2015.02.030. Epub 2015 Apr 30. Review. PMID: 25937611
  35. Typhoid fever in young children in Bangladesh: clinical findings, antibiotic susceptibility pattern and immune responses. Khanam F, Sayeed MA, Choudhury FK, Sheikh A, Ahmed D, Goswami D, Hossain ML, Brooks A, Calderwood SB, Charles RC, Cravioto A, Ryan ET, Qadri F. PLoS Negl Trop Dis. 2015 Apr 7;9(4):e0003619. doi: 10.1371/journal.pntd.0003619. eCollection 2015 Apr.
    PMID: 25849611
  36. Immunogenicity of the Bivalent Oral Cholera Vaccine Shanchol in Haitian Adults With HIV Infection.
  37. Ivers LC, Charles RC, Hilaire IJ, Mayo-Smith LM, Teng JE, Jerome JG, Rychert J, LaRocque RC, Xu P, Kováč P, Ryan ET, Qadri F, Almazor CP, Franke MF, Harris JB. J Infect Dis. 2015 Feb 26. pii: jiv108. [Epub ahead of print] PMID: 25722294
  38. Comparative proteomic analysis reveals activation of mucosal innate immune signaling pathways during cholera. Ellis CN, LaRocque RC, Uddin T, Krastins B, Mayo-Smith LM, Sarracino D, Karlsson EK, Rahman A, Shirin T, Bhuiyan TR, Chowdhury F, Khan AI, Ryan ET, Calderwood SB, Qadri F, Harris JB.
    Infect Immun. 2015 Mar;83(3):1089-103. doi: 10.1128/IAI.02765-14. Epub 2015 Jan 5. PMID: 25561705 [PubMed - indexed for MEDLINE]
  39. Household Transmission of Vibrio cholerae in Bangladesh. Sugimoto JD, Koepke AA, Kenah EE, Halloran ME, Chowdhury F, Khan AI, LaRocque RC, Yang Y, Ryan ET, Qadri F, Calderwood SB, Harris JB, Longini IM Jr. PLoS Negl Trop Dis. 2014 Nov 20;8(11):e3314. doi: 10.1371/journal.pntd.0003314. eCollection 2014 Nov. PMID: 25411971 [PubMed - in process]
  40. Evaluation of matrix-assisted laser desorption ionization-time of flight mass spectrometry for identification of Vibrio cholerae. Rychert J, Creely D, Mayo-Smith LM, Calderwood SB, Ivers LC, Ryan ET, Boncy J, Qadri F, Ahmed D, Ferraro MJ, Harris JB. J Clin Microbiol. 2015 Jan;53(1):329-31. doi: 10.1128/JCM.02666-14. Epub 2014 Nov 12. PMID: 25392355
  41. Environmental enteric dysfunction: pathogenesis, diagnosis, and clinical consequences. Keusch GT, Denno DM, Black RE, Duggan C, Guerrant RL, Lavery JV, Nataro JP, Rosenberg IH, Ryan ET, Tarr PI, Ward H, Bhutta ZA, Coovadia H, Lima A, Ramakrishna B, Zaidi AK, Hay Burgess DC, Brewer T.
    Clin Infect Dis. 2014 Nov 1;59 Suppl 4:S207-12. doi: 10.1093/cid/ciu485. Review. PMID: 25305288
  42. Hepatic hydatid cyst: a rare cause of recurrent pancreatitis. Kitchens WH, Liu C, Ryan ET, Fernandez-del Castillo C. J Gastrointest Surg. 2014 Nov;18(11):2057-9. doi: 10.1007/s11605-014-2630-1. Epub 2014 Aug 23. PMID: 25149853 [PubMed - indexed for MEDLINE]
  43. Circulating mucosal associated invariant T cells are activated in Vibrio cholerae O1 infection and associated with lipopolysaccharide antibody responses. Leung DT, Bhuiyan TR, Nishat NS, Hoq MR, Aktar A, Rahman MA, Uddin T, Khan AI, Chowdhury F, Charles RC, Harris JB, Calderwood SB, Qadri F, Ryan ET.
    PLoS Negl Trop Dis. 2014 Aug 21;8(8):e3076. doi: 10.1371/journal.pntd.0003076. eCollection 2014 Aug. PMID: 25144724 [PubMed - in process]
  44. Bacterial shedding in household contacts of cholera patients in Dhaka, Bangladesh. Weil AA, Begum Y, Chowdhury F, Khan AI, Leung DT, LaRocque RC, Charles RC, Ryan ET, Calderwood SB, Qadri F, Harris JB. Am J Trop Med Hyg. 2014 Oct;91(4):738-42. doi: 10.4269/ajtmh.14-0095. Epub 2014 Aug 11. PMID: 25114012 [PubMed - indexed for MEDLINE]
  45. Use of Japanese encephalitis vaccine in US travel medicine practices in Global TravEpiNet. Deshpande BR, Rao SR, Jentes ES, Hills SL, Fischer M, Gershman MD, Brunette GW, Ryan ET, LaRocque RC; Global TravEpiNet Consortium.
    Am J Trop Med Hyg. 2014 Oct;91(4):694-8. doi: 10.4269/ajtmh.14-0062. Epub 2014 Jul 28. PMID: 25070999 [PubMed - indexed for MEDLINE]
  46. Salmonella chronic carriage: epidemiology, diagnosis, and gallbladder persistence. Gunn JS, Marshall JM, Baker S, Dongol S, Charles RC, Ryan ET.
    Trends Microbiol. 2014 Nov;22(11):648-55. doi: 10.1016/j.tim.2014.06.007. Epub 2014 Jul 22. Review. PMID: 25065707 [PubMed - indexed for MEDLINE]
  47. Immunogenicity of a killed bivalent (O1 and O139) whole cell oral cholera vaccine, Shanchol, in Haiti. Charles RC, Hilaire IJ, Mayo-Smith LM, Teng JE, Jerome JG, Franke MF, Saha A, Yu Y, Kováč P, Calderwood SB, Ryan ET, LaRocque RC, Almazor CP, Qadri F, Ivers LC, Harris JB. PLoS Negl Trop Dis. 2014 May 1;8(5):e2828. doi: 10.1371/journal.pntd.0002828. eCollection 2014 May. PMID: 24786645 [PubMed - indexed for MEDLINE]
  48. Antigen-specific memory B-cell responses to enterotoxigenic Escherichia coli infection in Bangladeshi adults. Alam MM, Aktar A, Afrin S, Rahman MA, Aktar S, Uddin T, Rahman MA, Al Mahbuba D, Chowdhury F, Khan AI, Bhuiyan TR, Begum YA, Ryan ET, Calderwood SB, Svennerholm AM, Qadri F. PLoS Negl Trop Dis. 2014 Apr 24;8(4):e2822. doi: 10.1371/journal.pntd.0002822. eCollection 2014 Apr. PMID: 24762744 [PubMed - indexed for MEDLINE]
  49. Immune responses to O-specific polysaccharide and lipopolysaccharide of Vibrio cholerae O1 Ogawa in adult Bangladeshi recipients of an oral killed cholera vaccine and comparison to responses in patients with cholera. Uddin T, Aktar A, Xu P, Johnson RA, Rahman MA, Leung DT, Afrin S, Akter A, Alam MM, Rahman A, Chowdhury F, Khan AI, Bhuiyan TR, Bufano MK, Rashu R, Yu Y, Wu-Freeman Y, Harris JB, LaRocque RC, Charles RC, Kováč P, Calderwood SB, Ryan ET, Qadri F. Am J Trop Med Hyg. 2014 May;90(5):873-81. doi: 10.4269/ajtmh.13-0498. Epub 2014 Mar 31. PMID: 24686738 [PubMed - indexed for MEDLINE]
  50. Cellular and cytokine responses to Salmonella enterica serotype Typhi proteins in patients with typhoid fever in Bangladesh. Bhuiyan S, Sayeed A, Khanam F, Leung DT, Rahman Bhuiyan T, Sheikh A, Salma U, LaRocque RC, Harris JB, Pacek M, Calderwood SB, LaBaer J, Ryan ET, Qadri F, Charles RC. Am J Trop Med Hyg. 2014 Jun;90(6):1024-30. doi: 10.4269/ajtmh.13-0261. Epub 2014 Mar 10. PMID: 24615129
  51. Evaluation in mice of a conjugate vaccine for cholera made from Vibrio cholerae O1 (Ogawa) O-specific polysaccharide. Alam MM, Bufano MK, Xu P, Kalsy A, Yu Y, Freeman YW, Sultana T, Rashu MR, Desai I, Eckhoff G, Leung DT, Charles RC, LaRocque RC, Harris JB, Clements JD, Calderwood SB, Qadri F, Vann WF, Kováč P, Ryan ET. PLoS Negl Trop Dis. 2014 Feb 6;8(2):e2683. doi: 10.1371/journal.pntd.0002683. eCollection 2014 Feb. PMID: 24516685
  52. Pre-travel preparation of US travelers going abroad to provide humanitarian service, Global TravEpiNet 2009-2011. Stoney RJ, Jentes ES, Sotir MJ, Kozarsky P, Rao SR, Larocque RC, Ryan ET; Global TravEpiNet Consortium. Am J Trop Med Hyg. 2014 Mar;90(3):553-9. doi: 10.4269/ajtmh.13-0479. Epub 2014 Jan 20.
    PMID: 24445203
  53. Impact of Ramadan on Clinical and Microbiologic Parameters of Patients Seen at a Diarrheal Hospital in Urban Dhaka, Bangladesh, 1996-2012.  Leung DT, Das SK, Malek MA, Qadri F, Faruque AS, Ryan ET. Am J Trop Med Hyg. 2014 Jan 6. [Epub ahead of print] PMID: 24394479
  54. Immunoproteomic analysis of antibody-in-lymphocyte supernatant (ALS) in patients with typhoid fever in Bangladesh.  Charles RC, Liang L, Khanam F, Sayeed MA, Hung C, Leung DT, Baker S, Ludwig A, Harris JB, Larocque RC, Calderwood SB, Qadri F, Felgner PL, Ryan ET. Clin Vaccine Immunol. 2013 Dec 26. [Epub ahead of print] PMID: 24371257
  55. Implications of acquired environmental enteric dysfunction for growth and stunting in infants and children living in low- and middle-income countries.  Keusch GT, Rosenberg IH, Denno DM, Duggan C, Guerrant RL, Lavery JV, Tarr PI, Ward HD, Black RE, Nataro JP, Ryan ET, Bhutta ZA, Coovadia H, Lima A, Ramakrishna B, Zaidi AK, Burgess DC, Brewer T. Food Nutr Bull. 2013 Sep;34(3):357-64. Review. PMID: 24167916
  56. Eyes on the prize: lessons from the cholera wars for modern scientists, physicians, and public health officials.  Ryan ET. Am J Trop Med Hyg. 2013 Oct;89(4):610-4. doi: 10.4269/ajtmh.13-0173. PMID: 24106185
  57. The intestinal pathobiome: its reality and consequences among infants and young children in resource-limited settings.  Ryan ET. J Infect Dis. 2013 Dec 1;208(11):1732-3. doi: 10.1093/infdis/jit509. Epub 2013 Sep 16. PMID: 24041794
  58. Economics of Malaria Prevention in US Travelers to West Africa.  Adachi K, Coleman MS, Khan N, Jentes ES, Arguin P, Rao SR, Larocque RC, Sotir MJ, Brunette G, Ryan ET, Meltzer MI; Global TravEpiNet Consortium. Clin Infect Dis. 2014 Jan;58(1):11-21. doi: 10.1093/cid/cit570. Epub 2013 Sep 6. PMID: 24014735
  59. Evaluation of a typhoid/paratyphoid diagnostic assay (TPTest) detecting anti-Salmonella IgA in secretions of peripheral blood lymphocytes in patients in Dhaka, Bangladesh. Khanam F, Sheikh A, Sayeed MA, Bhuiyan MS, Choudhury FK, Salma U, Pervin S, Sultana T, Ahmed D, Goswami D, Hossain ML, Mamun KZ, Charles RC, Brooks WA, Calderwood SB, Cravioto A, Ryan ET, Qadri F. PMID: 23951368
  60. Antibody secreting cell responses after Vibrio cholerae O1 infection and oral cholera vaccination in adults in Bangladesh.  Rahman A, Rashu R, Bhuiyan TR, Chowdhury F, Khan AI, Islam K, Larocque RC, Ryan ET, Wrammert J, Calderwood SB, Qadri F, Harris JB. Clin Vaccine Immunol. 2013 Aug 14. [Epub ahead of print] PMID: 23945156
  61. Identification of Immunogenic Salmonella enterica Serotype Typhi Antigens Expressed in Chronic Biliary Carriers of S. Typhi in Kathmandu, Nepal.  Charles RC, Sultana T, Alam MM, Yu Y, Wu-Freeman Y, Bufano MK, Rollins SM, Tsai L, Harris JB, Larocque RC, Leung DT, Brooks WA, Nga TV, Dongol S, Basnyat B, Calderwood SB, Farrar J, Khanam F, Gunn JS, Qadri F, Baker S, Ryan ET. PLoS Negl Trop Dis. 2013 Aug 1;7(8):e2335. doi: 10.1371/journal.pntd.0002335. Print 2013 Aug. PMID: 23936575
  62. Antibody avidity following administration of an oral killed cholera vaccine: comparing humoral immune responses in Bangladeshi children with those in adults. Alam MM, Leung DT, Akhtar M, Nazim M, Akter S, Uddin T, Khanam F, Mahbuba DA, Ahmad SM, Bhuiyan TR, Calderwood SB, Ryan ET, Qadri F. Clin Vaccine Immunol. 2013 Aug 7. [Epub ahead of print] PMID: 23925888
  63. Evaluation of an electricity-free, culture-based approach for detecting typhoidal Salmonella bacteremia during enteric fever in a high burden, resource-limited setting. Andrews JR, Prajapati KG, Eypper E, Shrestha P, Shakya M, Pathak KR, Joshi N, Tiwari P, Risal M, Koirala S, Karkey A, Dongol S, Wen S, Smith AB, Maru D, Basnyat B, Baker S, Farrar J, Ryan ET, Hohmann E, Arjyal A. PLoS Negl Trop Dis. 2013 Jun 27;7(6):e2292. doi: 10.1371/journal.pntd.0002292. Print 2013 Jun. PMID: 23853696
  64. Natural selection in a bangladeshi population from the cholera-endemic ganges river delta.  Karlsson EK, Harris JB, Tabrizi S, Rahman A, Shlyakhter I, Patterson N, O'Dushlaine C, Schaffner SF, Gupta S, Chowdhury F, Sheikh A, Shin OS, Ellis C, Becker CE, Stuart LM, Calderwood SB, Ryan ET, Qadri F, Sabeti PC, Larocque RC. Sci Transl Med. 2013 Jul 3;5(192):192ra86. doi: 10.1126/scitranslmed.3006338. PMID: 23825302
  65. Immune responses to the O-specific polysaccharide antigen in children who received a killed oral cholera vaccine compared to responses following natural cholera infection in Bangladesh.  Leung DT, Uddin T, Xu P, Aktar A, Johnson RA, Rahman MA, Alam MM, Bufano MK, Eckhoff G, Wu-Freeman Y, Yu Y, Sultana T, Khanam F, Saha A, Chowdhury F, Khan AI, Charles RC, Larocque RC, Harris JB, Calderwood SB, Kovác P, Qadri F, Ryan ET. Clin Vaccine Immunol. 2013 Jun;20(6):780-8. doi: 10.1128/CVI.00035-13. Epub 2013 Mar 20. PMID: 23515016
  66. Identification of in vivo-induced bacterial proteins during human infection with Salmonella enterica serotype Paratyphi A. Alam MM, Tsai LL, Rollins SM, Sheikh A, Khanam F, Bufano MK, Yu Y, Wu-Freeman Y, Kalsy A, Sultana T, Sayeed MA, Jahan N, LaRocque RC, Harris JB, Leung DT, Brooks WA, Calderwood SB, Charles RC, Qadri F, Ryan ET. Clin Vaccine Immunol. 2013 May;20(5):712-9. doi: 10.1128/CVI.00054-13. Epub 2013 Mar 13. PMID: 23486419
  67. Travel characteristics and yellow fever vaccine usage among US Global TravEpiNet travelers visiting countries with risk of yellow fever virus transmission, 2009-2011. Jentes ES, Han P, Gershman MD, Rao SR, LaRocque RC, Staples JE, Ryan ET; Global TravEpiNet Consortium. Am J Trop Med Hyg. 2013 May;88(5):954-61. doi: 10.4269/ajtmh.12-0463. Epub 2013 Mar 4. PMID: 23458961
  68. Non-typhoidal Salmonella gastroenteritis at a diarrheal hospital in Dhaka, Bangladesh, 1996-2011. Leung DT, Das SK, Malek MA, Ahmed D, Khanam F, Qadri F, Faruque AS, Ryan ET. Am J Trop Med Hyg. 2013 Apr;88(4):661-9. doi: 10.4269/ajtmh.12-0672. Epub 2013 Jan 28. PMID: 2335864
  69. Pre-travel health care of immigrants returning home to visit friends and relatives. LaRocque RC, Deshpande BR, Rao SR, Brunette GW, Sotir MJ, Jentes ES, Ryan ET; Global TravEpiNet Consortium. Am J Trop Med Hyg. 2013 Feb;88(2):376-80. doi: 10.4269/ajtmh.2012.12-0460. Epub 2012 Nov 13. Erratum in: Am J Trop Med Hyg. 2013 Mar;88(3):606. PMID: 23149585
  70. Study of avidity of antigen-specific antibody as a means of understanding development of long-term immunological memory after Vibrio cholerae O1 infection. Alam MM, Arifuzzaman M, Ahmad SM, Hosen MI, Rahman MA, Rashu R, Sheikh A, Ryan ET, Calderwood SB, Qadri F. Clin Vaccine Immunol. 2013 Jan;20(1):17-23. doi: 10.1128/CVI.00521-12. Epub 2012 Oct 31. PMID: 23114701
  71. Robust microarray production of freshly expressed proteins in a human milieu. Festa F, Rollins SM, Vattem K, Hathaway M, Lorenz P, Mendoza EA, Yu X, Qiu J, Kilmer G, Jensen P, Webb B, Ryan ET, LaBaer J. Proteomics Clin Appl. 2013 Jun;7(5-6):372-7. doi: 10.1002/prca.201200063. Epub 2013 May 17. PMID: 23027544
  72. Comparison of immune responses to the O-specific polysaccharide and lipopolysaccharide of Vibrio cholerae O1 in Bangladeshi adult patients with cholera. Johnson RA, Uddin T, Aktar A, Mohasin M, Alam MM, Chowdhury F, Harris JB, LaRocque RC, Bufano MK, Yu Y, Wu-Freeman Y, Leung DT, Sarracino D, Krastins B, Charles RC, Xu P, Kovác P, Calderwood SB, Qadri F, Ryan ET. Clin Vaccine Immunol. 2012 Nov;19(11):1712-21. doi: 10.1128/CVI.00321-12. Epub 2012 Sep 19. PMID: 22993410
  73. Frequency of reexposure to Vibrio cholerae O1 evaluated by subsequent vibriocidal titer rise after an episode of severe cholera in a highly endemic area in Bangladesh. Weil AA, Chowdhury F, Khan AI, Leung DT, Uddin T, Begum YA, Saha NC, Charles RC, Larocque RC, Harris JB, Ryan ET, Qadri F, Calderwood SB. Am J Trop Med Hyg. 2012 Nov;87(5):921-6. doi: 10.4269/ajtmh.2012.12-0323. Epub 2012 Sep 10. PMID: 22964723
  74. High depth, whole-genome sequencing of cholera isolates from Haiti and the Dominican Republic. Sealfon R, Gire S, Ellis C, Calderwood S, Qadri F, Hensley L, Kellis M, Ryan ET, LaRocque RC, Harris JB, Sabeti PC. BMC Genomics. 2012 Sep 11;13:468. doi: 10.1186/1471-2164-13-468. PMID: 22963323
  75. Cholera.  Harris JB, LaRocque RC, Qadri F, Ryan ET, Calderwood SB. Lancet. 2012 Jun 30;379(9835):2466-76. PMID: 22748592
  76. Antigen-specific memory T cell responses after vaccination with an oral killed cholera vaccine in Bangladeshi children and comparison with natural cholera.
    Arifuzzaman M, Rashu R, Leung DT, Hosen MI, Bhuiyan TR, Bhuiyan MS, Rahman MA, Khanam F, Saha A, Charles RC, Larocque RC, Weil AA, Clements JD, Holmes RK, Calderwood SB, Harris JB, Ryan ET, Qadri F. Clin Vaccine Immunol. 2012 Jun 27. [Epub ahead of print] PMID: 22739692
  77. <aclass="link-badge--external" href="http://www.ncbi.nlm.nih.gov/pubmed/22665823">Travel medicine.  Schwartz BS, Larocque RC, Ryan ET. Ann Intern Med. 2012 Jun 5;156(11):ITC61. PMID: 22665823
  78. Memory B cell responses to Vibrio cholerae O1 lipopolysaccharide are associated with protection against infection from household contacts of patients with cholera in Bangladesh.  Patel SM, Rahman MA, Mohasin M, Riyadh MA, Leung DT, Alam MM, Chowdhury F, Khan AI, Weil AA, Aktar A, Nazim M, LaRocque RC, Ryan ET, Calderwood SB, Qadri F, Harris JB. Clin Vaccine Immunol. 2012 Jun;19(6):842-8. Epub 2012 Apr 18. PMID: 22518009
  79. Immune responses to cholera in children.  Leung DT, Chowdhury F, Calderwood SB, Qadri F, Ryan ET. Expert Rev Anti Infect Ther. 2012 Apr;10(4):435-44. PMID: 22512753
  80. Factors associated with encephalopathy in patients with Salmonella enterica serotype Typhi bacteremia presenting to a diarrheal hospital in Dhaka, Bangladesh.  Leung DT, Bogetz J, Itoh M, Ganapathi L, Pietroni MA, Ryan ET, Chisti MJ. Am J Trop Med Hyg. 2012 Apr;86(4):698-702. PMID: 22492156
  81. Memory B cell and other immune responses in children receiving two doses of an oral killed cholera vaccine compared to responses following natural cholera infection in Bangladesh.
    Leung DT, Rahman MA, Mohasin M, Patel SM, Aktar A, Khanam F, Uddin T, Riyadh MA, Saha A, Alam MM, Chowdhury F, Khan AI, Charles R, LaRocque R, Harris JB, Calderwood SB, Qadri F, Ryan ET. Clin Vaccine Immunol. 2012 May;19(5):690-8. Epub 2012 Mar 21. PMID: 22441386
  82. Transcutaneous immunization with a Vibrio cholerae O1 Ogawa synthetic hexasaccharide conjugate following oral whole-cell cholera vaccination boosts vibriocidal responses and induces protective immunity in mice.  Tarique AA, Kalsy A, Arifuzzaman M, Rollins SM, Charles RC, Leung DT, Harris JB, Larocque RC, Sheikh A, Bhuiyan MS, Saksena R, Clements JD, Calderwood SB, Qadri F, Kovác P, Ryan ET. Clin Vaccine Immunol. 2012 Apr;19(4):594-602. Epub 2012 Feb 22. PMID: 22357651
  83. Systemic antibody responses to the immunodominant p23 antigen and p23 polymorphisms in children with cryptosporidiosis in Bangladesh.  Borad AJ, Allison GM, Wang D, Ahmed S, Karim MM, Kane AV, Moy J, Hibberd PL, Ajjampur SS, Kang G, Calderwood SB, Ryan ET, Naumova E, Khan WA, Ward HD. Am J Trop Med Hyg. 2012 Feb;86(2):214-22. PMID: 22302851
  84. Individuals with Le(a+b-) blood group have increased susceptibility to symptomatic vibrio cholerae O1 infection.  Arifuzzaman M, Ahmed T, Rahman MA, Chowdhury F, Rashu R, Khan AI, LaRocque RC, Harris JB, Bhuiyan TR, Ryan ET, Calderwood SB, Qadri F. PLoS Negl Trop Dis. 2011 Dec;5(12):e1413. doi: 10.1371/journal.pntd.0001413. Epub 2011 Dec 27. PMID: 22216364 Free PMC Article
  85. Haiti in the context of the current global cholera pandemic.  Ryan ET. Emerg Infect Dis. 2011 Nov;17(11):2175-6. PMID: 22204041 Free PMC Article
  86. In vivo expression of Salmonella enterica serotype Typhi genes in the blood of patients with typhoid fever in Bangladesh.  Sheikh A, Charles RC, Sharmeen N, Rollins SM, Harris JB, Bhuiyan MS, Arifuzzaman M, Khanam F, Bukka A, Kalsy A, Porwollik S, Leung DT, Brooks WA, LaRocque RC, Hohmann EL, Cravioto A, Logvinenko T, Calderwood SB, McClelland M, Graham JE, Qadri F, Ryan ET. PLoS Negl Trop Dis. 2011 Dec;5(12):e1419. Epub 2011 Dec 13. PMID: 22180799 Free PMC Article
  87. Case records of the Massachusetts General Hospital. Case 38-2011. A 34-year-old man with diarrhea and weakness.  Ryan ET, Cronin CG, Branda JA. N Engl J Med. 2011 Dec 15;365(24):2306-16. PMID: 22168646
  88. Global TravEpiNet: a national consortium of clinics providing care to international travelers--analysis of demographic characteristics, travel destinations, and pretravel healthcare of high-risk US international travelers, 2009-2011.  LaRocque RC, Rao SR, Lee J, Ansdell V, Yates JA, Schwartz BS, Knouse M, Cahill J, Hagmann S, Vinetz J, Connor BA, Goad JA, Oladele A, Alvarez S, Stauffer W, Walker P, Kozarsky P, Franco-Paredes C, Dismukes R, Rosen J, Hynes NA, Jacquerioz F, McLellan S, Hale D, Sofarelli T, Schoenfeld D, Marano N, Brunette G, Jentes ES, Yanni E, Sotir MJ, Ryan ET; Global TravEpiNet Consortium. Clin Infect Dis. 2012 Feb 15;54(4):455-62. Epub 2011 Dec 5. PMID: 22144534
  89. LPLUNC1 modulates innate immune responses to Vibrio cholerae.  Shin OS, Uddin T, Citorik R, Wang JP, Della Pelle P, Kradin RL, Bingle CD, Bingle L, Camilli A, Bhuiyan TR, Shirin T, Ryan ET, Calderwood SB, Finberg RW, Qadri F, Larocque RC, Harris JB. J Infect Dis. 2011 Nov;204(9):1349-57. Epub 2011 Sep 7. PMID: 21900486
  90. Simple, direct conjugation of bacterial O-SP-core antigens to proteins: development of cholera conjugate vaccines.  Xu P, Alam MM, Kalsy A, Charles RC, Calderwood SB, Qadri F, Ryan ET, Kováč P. Bioconjug Chem. 2011 Oct 19;22(10):2179-85. Epub 2011 Sep 30. PMID: 21899371
  91. Cholera in the 21st century.  Charles RC, Ryan ET. Curr Opin Infect Dis. 2011 Oct;24(5):472-7. Review. PMID: 21799407
  92. Antibody responses to the immunodominant Cryptosporidium gp15 antigen and gp15 polymorphisms in a case-control study of cryptosporidiosis in children in Bangladesh.  Allison GM, Rogers KA, Borad A, Ahmed S, Karim MM, Kane AV, Hibberd PL, Naumova EN, Calderwood SB, Ryan ET, Khan WA, Ward HD. Am J Trop Med Hyg. 2011 Jul;85(1):97-104. PMID: 21734132 Free PMC Article
  93. Case records of the Massachusetts General Hospital. Case 20-2011. A 30-year-old man with diarrhea after a trip to the Dominican Republic. Ryan ET, Madoff LC, Ferraro MJ. N Engl J Med. 2011 Jun 30;364(26):2536-41. PMID: 21714651
  94. Vibrio cholerae O1 infection induces proinflammatory CD4+ T-cell responses in blood and intestinal mucosa of infected humans.  Kuchta A, Rahman T, Sennott EL, Bhuyian TR, Uddin T, Rashu R, Chowdhury F, Kahn AI, Arifuzzaman M, Weil AA, Podolsky M, LaRocque RC, Ryan ET, Calderwood SB, Qadri F, Harris JB. Clin Vaccine Immunol. 2011 Aug;18(8):1371-7. Epub 2011 Jun 22. PMID: 21697339 Free PMC Article
  95. Comparison of memory B cell, antibody-secreting cell, and plasma antibody responses in young children, older children, and adults with infection caused by Vibrio cholerae O1 El Tor Ogawa in Bangladesh.  Leung DT, Rahman MA, Mohasin M, Riyadh MA, Patel SM, Alam MM, Chowdhury F, Khan AI, Kalivoda EJ, Aktar A, Bhuiyan MS, LaRocque RC, Harris JB, Calderwood SB, Qadri F, Ryan ET. Clin Vaccine Immunol. 2011 Aug;18(8):1317-25. Epub 2011 Jun 22. PMID: 21697337 Free PMC Article
  96. Interferon-γ and proliferation responses to Salmonella enterica Serotype Typhi proteins in patients with S. Typhi Bacteremia in Dhaka, Bangladesh.  Sheikh A, Khanam F, Sayeed MA, Rahman T, Pacek M, Hu Y, Rollins A, Bhuiyan MS, Rollins S, Kalsy A, Arifuzzaman M, Leung DT, Sarracino DA, Krastins B, Charles RC, Larocque RC, Cravioto A, Calderwood SB, Brooks WA, Harris JB, Labaer J, Qadri F, Ryan ET. PLoS Negl Trop Dis. 2011 Jun;5(6):e1193. Epub 2011 Jun 7. PMID: 21666798 Free PMC Article
  97. Meeting cholera's challenge to Haiti and the world: a joint statement on cholera prevention and care.  Farmer P, Almazor CP, Bahnsen ET, Barry D, Bazile J, Bloom BR, Bose N, Brewer T, Calderwood SB, Clemens JD, Cravioto A, Eustache E, Jérôme G, Gupta N, Harris JB, Hiatt HH, Holstein C, Hotez PJ, Ivers LC, Kerry VB, Koenig SP, Larocque RC, Léandre F, Lambert W, Lyon E, Mekalanos JJ, Mukherjee JS, Oswald C, Pape JW, Gretchko Prosper A, Rabinovich R, Raymonville M, Réjouit JR, Ronan LJ, Rosenberg ML, Ryan ET, Sachs JD, Sack DA, Surena C, Suri AA, Ternier R, Waldor MK, Walton D, Weigel JL. PLoS Negl Trop Dis. 2011;5(5):e1145. Epub 2011 May 31. Review. PMID: 21655350 Free PMC Article
  98. Genetic diversity of Cryptosporidium spp. from Bangladeshi children. Hira KG, Mackay MR, Hempstead AD, Ahmed S, Karim MM, O'Connor RM, Hibberd PL, Calderwood SB, Ryan ET, Khan WA, Ward HD. J Clin Microbiol. 2011 Jun;49(6):2307-10. Epub 2011 Apr 6. PMID: 21471344 Free PMC Article
  99. Antigen-specific memory B-cell responses in Bangladeshi adults after one or two dose oral killed cholera vaccination, and comparison with responses following natural cholera.
    Alam MM, Riyadh MA, Fatema K, Rahman MA, Akhtar N, Ahmed T, Chowdhury MI, Chowdhury F, Calderwood SB, Harris JB, Ryan ET, Qadri F.Clin Vaccine Immunol. 2011 Feb 23. [Epub ahead of print] PMID: 21346055
  100. The cholera pandemic, still with us after half a century: time to rethink. Ryan ET. PLoS Negl Trop Dis. 2011 Jan 25;5(1):e1003.  PMID: 21283611
  101. Mucosal immunologic responses in cholera patients in bangladesh. Uddin T, Harris JB, Bhuiyan TR, Shirin T, Uddin MI, Khan AI, Chowdhury F, Larocque RC, Alam NH, Ryan ET, Calderwood SB, Qadri F.Clin Vaccine Immunol. 2011 Mar;18(3):506-12. Epub 2011 Jan 19. PMID: 21248157
  102. Severe, acute watery diarrhea in an adult. Chowdhury F, Khan AI, Faruque AS, Ryan ET. PLoS Negl Trop Dis. 2010 Nov 30;4(11):e898.  PMID: 21152053
  103. Analysis of Salmonella enterica serotype paratyphi A gene expression in the blood of bacteremic patients in Bangladesh. Sheikh A, Charles RC, Rollins SM, Harris JB, Bhuiyan MS, Khanam F, Bukka A, Kalsy A, Porwollik S, Brooks WA, LaRocque RC, Hohmann EL, Cravioto A, Logvinenko T, Calderwood SB, McClelland M, Graham JE, Qadri F, Ryan ET. PLoS Negl Trop Dis. 2010 Dec 7;4(12):e908. PMID: 21151879
  104. Relatedness of Vibrio cholerae O1/O139 isolates from patients and their household contacts, determined by multilocus variable-number tandem-repeat analysis. Kendall EA, Chowdhury F, Begum Y, Khan AI, Li S, Thierer JH, Bailey J, Kreisel K, Tacket CO, LaRocque RC, Harris JB, Ryan ET, Qadri F, Calderwood SB, Stine OC. J Bacteriol. 2010 Sep;192(17):4367-76. Epub 2010 Jun 28. PMID: 20585059
  105. Characterization of anti-Salmonella enterica serotype Typhi antibody responses in bacteremic Bangladeshi patients by an immunoaffinity proteomics-based technology. Charles RC, Sheikh A, Krastins B, Harris JB, Bhuiyan MS, LaRocque RC, Logvinenko T, Sarracino DA, Kudva IT, Eisenstein J, Podolsky MJ, Kalsy A, Brooks WA, Ludwig A, John M, Calderwood SB, Qadri F, Ryan ET.Clin Vaccine Immunol. 2010 Aug;17(8):1188-95. Epub 2010 Jun 23. PMID: 20573880
  106. Informal urban settlements and cholera risk in Dar es Salaam, Tanzania. Penrose K, de Castro MC, Werema J, Ryan ET.PLoS Negl Trop Dis. 2010 Mar 16;4(3):e631. PMID: 20300569
  107. Concomitant enterotoxigenic Escherichia coli infection induces increased immune responses to Vibrio cholerae O1 antigens in patients with cholera in Bangladesh.
    Chowdhury F, Begum YA, Alam MM, Khan AI, Ahmed T, Bhuiyan MS, Harris JB, LaRocque RC, Faruque AS, Endtz H, Ryan ET, Cravioto A, Svennerholm AM, Calderwood SB, Qadri F. Infect Immun. 2010 May;78(5):2117-24. Epub 2010 Feb 22. PMID: 20176796
  108. Familial aggregation of Vibrio cholerae-associated infection in Matlab, Bangladesh. Rahman KM, Duggal P, Harris JB, Saha SK, Streatfield PK, Ryan ET, Calderwood SB, Qadri F, Yunus M, LaRocque RC.J Health Popul Nutr. 2009 Dec;27(6):733-8. PMID: 20099756
  109. Comparison of clinical features and immunological parameters of patients with dehydrating diarrhoea infected with Inaba or Ogawa serotypes of Vibrio cholerae O1. Khan AI, Chowdhury F, Harris JB, Larocque RC, Faruque AS, Ryan ET, Calderwood SB, Qadri F. Scand J Infect Dis. 2009 Nov 2. PMID: 19883159
  110. Development of IgM memory to both a T cell-independent and a T cell-dependent antigen following infection with Vibrio cholerae O1 in Bangladesh. Kendall EA, Tarique AA, Hossain A, Alam MM, Arifuzzaman M, Akhtar N, Chowdhury F, Khan AI, Larocque RC, Harris JB, Ryan ET, Qadri F, Calderwood SB.
    Infect Immun. 2009 Oct 26. PMID: 19858296
  111. Clinical outcomes in household contacts of patients with cholera in bangladesh. Weil AA, Khan AI, Chowdhury F, Larocque RC, Faruque AS, Ryan ET, Calderwood SB, Qadri F, Harris JB. Clin Infect Dis. 2009 Nov 15;49(10):1473-9.PMID: 19842974
  112. Comparative proteomic analysis of the PhoP regulon in Salmonella enterica serovar Typhi versus Typhimurium. Charles RC, Harris JB, Chase MR, Lebrun LM, Sheikh A, LaRocque RC, Logvinenko T, Rollins SM, Tarique A, Hohmann EL, Rosenberg I, Krastins B, Sarracino DA, Qadri F, Calderwood SB, Ryan ET. PLoS One. 2009 Sep 10;4(9):e6994.PMID: 19746165 Free article
  113. Salmonella enterica serovar Typhi-specific immunoglobulin A antibody responses in plasma and antibody in lymphocyte supernatant specimens in Bangladeshi patients with suspected typhoid fever. Sheikh A, Bhuiyan MS, Khanam F, Chowdhury F, Saha A, Ahmed D, Jamil KM, LaRocque RC, Harris JB, Ahmad MM, Charles R, Brooks WA, Calderwood SB, Cravioto A, Ryan ET, Qadri F. Clin Vaccine Immunol. 2009 Nov;16(11):1587-94. Epub 2009 Sep 9.PMID: 19741090
  114. Memory T-cell responses to Vibrio cholerae O1 infection. Weil AA, Arifuzzaman M, Bhuiyan TR, LaRocque RC, Harris AM, Kendall EA, Hossain A, Tarique AA, Sheikh A, Chowdhury F, Khan AI, Murshed F, Parker KC, Banerjee KK, Ryan ET, Harris JB, Qadri F, Calderwood SB. Infect Immun. 2009 Nov;77(11):5090-6. Epub 2009 Aug 24.PMID: 19703973
  115. Vaccination of children in low-resource countries against Shigella is unlikely to present an undue risk of reactive arthritis. Gaston JS, Inman RD, Ryan ET, Venkatesan MM, Barry EM, Hale TL, Bourgeois AL, Walker RI. Vaccine. 2009 Sep 4;27(40):5432-4. Epub 2009 Jul 28.PMID:
  116. Transcutaneous immunization with a synthetic hexasaccharide-protein conjugate induces anti-Vibrio cholerae lipopolysaccharide responses in mice. Rollenhagen JE, Kalsy A, Saksena R, Sheikh A, Alam MM, Qadri F, Calderwood SB, Kovác P, Ryan ET. Vaccine. 2009 Aug 6;27(36):4917-22. Epub 2009 Jun 27.PMID: 19563890
  117. Antigen-specific memory B-cell responses to Vibrio cholerae O1 infection in Bangladesh. Harris AM, Bhuiyan MS, Chowdhury F, Khan AI, Hossain A, Kendall EA, Rahman A, LaRocque RC, Wrammert J, Ryan ET, Qadri F, Calderwood SB, Harris JB.
    Infect Immun. 2009 Sep;77(9):3850-6. Epub 2009 Jun 15.PMID: 19528207
  118. Immunologic Responses to Vibrio cholerae in Patients Co-Infected with Intestinal Parasites in Bangladesh. Harris JB, Podolsky MJ, Bhuiyan TR, Chowdhury F, Khan AI, Larocque RC, Logvinenko T, Kendall J, Faruque AS, Nagler CR, Ryan ET, Qadri F, Calderwood SB. PLoS Negl Trop Dis. 2009;3(3):e403. Epub 2009 Mar 31.PMID: 19333369 Free article
  119. A variant in long palate, lung and nasal epithelium clone 1 is associated with cholera in a Bangladeshi population. Larocque RC, Sabeti P, Duggal P, Chowdhury F, Khan AI, Lebrun LM, Harris JB, Ryan ET, Qadri F, Calderwood SB. Genes Immun. 2009 Apr;10(3):267-72. Epub 2009 Feb 12.PMID: 19212328 Free article
  120. Shifting prevalence of major diarrheal pathogens in patients seeking hospital care during floods in 1998, 2004, and 2007 in Dhaka, Bangladesh.
    Harris AM, Chowdhury F, Begum YA, Khan AI, Faruque AS, Svennerholm AM, Harris JB, Ryan ET, Cravioto A, Calderwood SB, Qadri F. Am J Trop Med Hyg. 2008 Nov;79(5):708-14.PMID: 18981509 Free article
  121. Management of travellers' diarrhoea. Hill DR, Ryan ET. BMJ. 2008 Oct 6;337:a1746. doi: 10.1136/bmj.a1746. Review. PMID: 18838421
  122. A comparison of clinical and immunologic features in children and older patients hospitalized with severe cholera in Bangladesh.
    Chowdhury F, Khan AI, Harris JB, LaRocque RC, Chowdhury MI, Ryan ET, Faruque AS, Calderwood SB, Qadri F. Pediatr Infect Dis J. 2008 Nov;27(11):986-92.PMID: 18833030 Free article
  123. Cholera toxin-specific memory B cell responses are induced in patients with dehydrating diarrhea caused by Vibrio cholerae O1. Jayasekera CR, Harris JB, Bhuiyan S, Chowdhury F, Khan AI, Faruque AS, Larocque RC, Ryan ET, Ahmed R, Qadri F, Calderwood SB. J Infect Dis. 2008 Oct 1;198(7):1055-61.PMID: 18729782 Free article
  124. Proteomic analysis of Vibrio cholerae in human stool. LaRocque RC, Krastins B, Harris JB, Lebrun LM, Parker KC, Chase M, Ryan ET, Qadri F, Sarracino D, Calderwood SB. Infect Immun. 2008 Sep;76(9):4145-51. Epub 2008 Jun 30.PMID: 18591230 Free article
  125. Susceptibility to Vibrio cholerae Infection in a Cohort of Household Contacts of Patients with Cholera in Bangladesh. Harris JB, Larocque RC, Chowdhury F, Khan AI, Logvinenko T, Faruque AS, Ryan ET, Qadri F, Calderwood SB. PLoS Negl Trop Dis. 2008 Apr 9;2(4):e221.PMID: 18398491 Free article
  126. Application of in vivo induced antigen technology (IVIAT) to Bacillus anthracis. Rollins SM, Peppercorn A, Young JS, Drysdale M, Baresch A, Bikowski MV, Ashford DA, Quinn CP, Handfield M, Hillman JD, Lyons CR, Koehler TM, Calderwood SB, Ryan ET. PLoS One. 2008 Mar 19;3(3):e1824.PMID: 18350160 Free article
  127. Complexity of rice-water stool from patients with Vibrio cholerae plays a role in the transmission of infectious diarrhea. Nelson EJ, Chowdhury A, Harris JB, Begum YA, Chowdhury F, Khan AI, Larocque RC, Bishop AL, Ryan ET, Camilli A, Qadri F, Calderwood SB. Proc Natl Acad Sci U S A. 2007 Nov 27;104(48):19091-6. Epub 2007 Nov 16.PMID: 18024592 Free article
  128. Case records of the Massachusetts General Hospital. Case 29-2007. A 51-year-old man with gastric cancer and lung nodules. Ryan ET, Aquino SL, Kradin RL. N Engl J Med. 2007 Sep 20;357(12):1239-46. PMID: 17881756
  129. Bacteremia, fever, and splenomegaly caused by a newly recognized bartonella species. Eremeeva ME, Gerns HL, Lydy SL, Goo JS, Ryan ET, Mathew SS, Ferraro MJ, Holden JM, Nicholson WL, Dasch GA, Koehler JE.
    N Engl J Med. 2007 Jun 7;356(23):2381-7.PMID: 17554119 Free article
  130. Transcutaneous immunization with Clostridium difficile toxoid A induces systemic and mucosal immune responses and toxin A-neutralizing antibodies in mice. Ghose C, Kalsy A, Sheikh A, Rollenhagen J, John M, Young J, Rollins SM, Qadri F, Calderwood SB, Kelly CP, Ryan ET. Infect Immun. 2007 Jun;75(6):2826-32. Epub 2007 Mar 19.PMID: 17371854 Free article
  131. The practice of travel medicine: guidelines by the Infectious Diseases Society of America. Hill DR, Ericsson CD, Pearson RD, Keystone JS, Freedman DO, Kozarsky PE, DuPont HL, Bia FJ, Fischer PR, Ryan ET; Infectious Diseases Society of America. Clin Infect Dis. 2006 Dec 15;43(12):1499-539. Epub 2006 Nov 8. PMID: 17109284
  132. Live attenuated oral cholera vaccines. Ryan ET, Calderwood SB, Qadri F.
    Expert Rev Vaccines. 2006 Aug;5(4):483-94. Review.PMID: 16989629
  133. Transcutaneous immunization with toxin-coregulated pilin A induces protective immunity against Vibrio cholerae O1 El Tor challenge in mice. Rollenhagen JE, Kalsy A, Cerda F, John M, Harris JB, Larocque RC, Qadri F, Calderwood SB, Taylor RK, Ryan ET. Infect Immun. 2006 Oct;74(10):5834-9.PMID: 16988262 Free article
  134. Primaquine: report from CDC expert meeting on malaria chemoprophylaxis I. Hill DR, Baird JK, Parise ME, Lewis LS, Ryan ET, Magill AJ. Am J Trop Med Hyg. 2006 Sep;75(3):402-15.PMID: 16968913 Free article
  135. Infectious diseases of severe weather-related and flood-related natural disasters. Ivers LC, Ryan ET. Curr Opin Infect Dis. 2006 Oct;19(5):408-14. Review.PMID: 16940862
  136. Identification of in vivo-induced bacterial protein antigens during human infection with Salmonella enterica serovar Typhi. Harris JB, Baresch-Bernal A, Rollins SM, Alam A, LaRocque RC, Bikowski M, Peppercorn AF, Handfield M, Hillman JD, Qadri F, Calderwood SB, Hohmann E, Breiman RF, Brooks WA, Ryan ET. Infect Immun. 2006 Sep;74(9):5161-8.PMID: 16926408 Free article
  137. Postgenomic approaches to cholera vaccine development. LaRocque RC, Harris JB, Ryan ET, Qadri F, Calderwood SB. Expert Rev Vaccines. 2006 Jun;5(3):337-46. Review.PMID: 16827618
  138. Diarrheal epidemics in Dhaka, Bangladesh, during three consecutive floods: 1988, 1998, and 2004. Schwartz BS, Harris JB, Khan AI, Larocque RC, Sack DA, Malek MA, Faruque AS, Qadri F, Calderwood SB, Luby SP, Ryan ET.
    Am J Trop Med Hyg. 2006 Jun;74(6):1067-73.PMID: 16760521 Free article
  139. Low risk of hemolytic uremic syndrome after early effective antimicrobial therapy for Shigella dysenteriae type 1 infection in Bangladesh. Bennish ML, Khan WA, Begum M, Bridges EA, Ahmed S, Saha D, Salam MA, Acheson D, Ryan ET. Clin Infect Dis. 2006 Feb 1;42(3):356-62. Epub 2005 Dec 21.PMID: 16392080
  140. Case records of the Massachusetts General Hospital. Case 39-2005. A 63-year-old woman with a positive serologic test for syphilis and persistent eosinophilia. Ryan ET, Felsenstein D, Aquino SL, Branda JA, Morgan JG. N Engl J Med. 2005 Dec 22;353(25):2697-705. PMID: 16371636
  141. Blood group, immunity, and risk of infection with Vibrio cholerae in an area of endemicity. Harris JB, Khan AI, LaRocque RC, Dorer DJ, Chowdhury F, Faruque AS, Sack DA, Ryan ET, Qadri F, Calderwood SB. Infect Immun. 2005 Nov;73(11):7422-7.PMID: 16239542 Free article
  142. Hyperinfectivity of human-passaged Vibrio cholerae can be modeled by growth in the infant mouse. Alam A, Larocque RC, Harris JB, Vanderspurt C, Ryan ET, Qadri F, Calderwood SB. Infect Immun. 2005 Oct;73(10):6674-9.PMID: 16177344 Free article
  143. Reduction in capsular content and enhanced bacterial susceptibility to serum killing of Vibrio cholerae O139 associated with the 2002 cholera epidemic in Bangladesh. Qadri F, Svennerholm AM, Shamsuzzaman S, Bhuiyan TR, Harris JB, Ghosh AN, Nair GB, Weintraub A, Faruque SM, Ryan ET, Sack DA, Calderwood SB. Infect Immun. 2005 Oct;73(10):6577-83.PMID: 16177333 Free article
  144. Species-specific bacteria identification using differential mobility spectrometry and bioinformatics pattern recognition. Shnayderman M, Mansfield B, Yip P, Clark HA, Krebs MD, Cohen SJ, Zeskind JE, Ryan ET, Dorkin HL, Callahan MV, Stair TO, Gelfand JA, Gill CJ, Hitt B, Davis CE. Anal Chem. 2005 Sep 15;77(18):5930-7.PMID: 16159124
  145. Transcriptional profiling of Vibrio cholerae recovered directly from patient specimens during early and late stages of human infection. Larocque RC, Harris JB, Dziejman M, Li X, Khan AI, Faruque AS, Faruque SM, Nair GB, Ryan ET, Qadri F, Mekalanos JJ, Calderwood SB. Infect Immun. 2005 Aug;73(8):4488-93.PMID: 16040959 Free article
  146. Transformation of the developing world: socioeconomic matrix. Carroll D, Gardner P, Kay BA, Osterholm M, Ryan ET. Emerg Infect Dis. 2004 Nov;10(11):2049. PMID: 16010742
  147. Case records of the Massachusetts General Hospital. Case 19-2005. A 17-year-old girl with respiratory distress and hemiparesis after surviving a tsunami. Kao AY, Munandar R, Ferrara SL, Systrom DM, Sheridan RL, Cash SS, Ryan ET. N Engl J Med. 2005 Jun 23;352(25):2628-36. PMID: 15972870
  148. Prevention of infection in adult travelers after solid organ transplantation. Kotton CN, Ryan ET, Fishman JA. Am J Transplant. 2005 Jan;5(1):8-14. Review.PMID: 15636606
  149. In vivo induced antigen technology (IVIAT). Rollins SM, Peppercorn A, Hang L, Hillman JD, Calderwood SB, Handfield M, Ryan ET. Cell Microbiol. 2005 Jan;7(1):1-9. Review.PMID: 15617518
  150. Cryptosporidiosis among Bangladeshi children with diarrhea: a prospective, matched, case-control study of clinical features, epidemiology and systemic antibody responses. Khan WA, Rogers KA, Karim MM, Ahmed S, Hibberd PL, Calderwood SB, Ryan ET, Ward HD. Am J Trop Med Hyg. 2004 Oct;71(4):412-9.PMID: 15516636 Free article
  151. The major subunit of the toxin-coregulated pilus TcpA induces mucosal and systemic immunoglobulin A immune responses in patients with cholera caused by Vibrio cholerae O1 and O139. Asaduzzaman M, Ryan ET, John M, Hang L, Khan AI, Faruque AS, Taylor RK, Calderwood SB, Qadri F. Infect Immun. 2004 Aug;72(8):4448-54.PMID: 15271902 Free article
  152. Incomplete correlation of serum vibriocidal antibody titer with protection from Vibrio cholerae infection in urban Bangladesh. Saha D, LaRocque RC, Khan AI, Harris JB, Begum YA, Akramuzzaman SM, Faruque AS, Ryan ET, Qadri F, Calderwood SB. J Infect Dis. 2004 Jun 15;189(12):2318-22. Epub 2004 May 25.PMID: 15181581
  153. Medical problems in the returning expatriate. Hochberg N, Ryan ET. Clin Occup Environ Med. 2004 Feb;4(1):205-19. Review.PMID: 15043371
  154. Vaccination strategies. Ivers LC, Ryan ET. Clin Occup Environ Med. 2004 Feb;4(1):27-43.PMID: 15043362
  155. Yersinia pestis and the plague. Rollins SE, Rollins SM, Ryan ET. Am J Clin Pathol. 2003 Jun;119 Suppl:S78-85. Review.PMID: 12951845
  156. Antigen-specific immunoglobulin A antibodies secreted from circulating B cells are an effective marker for recent local immune responses in patients with cholera: comparison to antibody-secreting cell responses and other immunological markers. Qadri F, Ryan ET, Faruque AS, Ahmed F, Khan AI, Islam MM, Akramuzzaman SM, Sack DA, Calderwood SB. Infect Immun. 2003 Aug;71(8):4808-14.PMID: 12874365 Free article
  157. Use of in vivo-induced antigen technology (IVIAT) to identify genes uniquely expressed during human infection with Vibrio cholerae. Hang L, John M, Asaduzzaman M, Bridges EA, Vanderspurt C, Kirn TJ, Taylor RK, Hillman JD, Progulske-Fox A, Handfield M, Ryan ET, Calderwood SB. Proc Natl Acad Sci U S A. 2003 Jul 8;100(14):8508-13. Epub 2003 Jun 25.PMID: 12826608 Free article
  158. Images in clinical medicine. Thoracic echinococcosis. Brainard DM, Ryan ET. N Engl J Med. 2003 Feb 6;348(6):528. PMID: 12571260
  159. Illness after international travel. Ryan ET, Wilson ME, Kain KC. N Engl J Med. 2002 Aug 15;347(7):505-16. Review. PMID: 12181406
  160. Case records of the Massachusetts General Hospital. Weekly clinicopathological exercises. Case 20-2002. A 37-year-old man with fever, hepatosplenomegaly, and a cutaneous foot lesion after a trip to Africa. Moore AC, Ryan ET, Waldron MA. N Engl J Med. 2002 Jun 27;346(26):2069-76. PMID: 12087144
  161. Comparison of mucosal and systemic humoral immune responses after transcutaneous and oral immunization strategies. John M, Bridges EA, Miller AO, Calderwood SB, Ryan ET. Vaccine. 2002 Jun 21;20(21-22):2720-6.PMID: 12034098
  162. Malaria: epidemiology, pathogenesis, diagnosis, prevention, and treatment--an update. Ryan ET. Curr Clin Top Infect Dis. 2001;21:83-113. Review. PMID: 11572163
  163. Local production of anti-vibrio cholerae mucosal antibody in reproductive tract tissues after cholera. Ryan ET, Bridges EA, Crean TI, Gausia K, Hamadani JD, Aziz A, Hawkes S, Begum M, Bogaerts J, Faruque SM, Salam MA, Fuchs GJ, Calderwood SB. J Infect Dis. 2001 Sep 1;184(5):643-7. Epub 2001 Aug 9.PMID: 11494170
  164. Mortality, morbidity, and microbiology of endemic cholera among hospitalized patients in Dhaka, Bangladesh. Ryan ET, Dhar U, Khan WA, Salam MA, Faruque AS, Fuchs GJ, Calderwood SB, Bennish ML. Am J Trop Med Hyg. 2000 Jul-Aug;63(1-2):12-20.PMID: 11357989 Free article
  165. Cholera vaccines. Ryan ET, Calderwood SB. J Travel Med. 2001 Mar-Apr;8(2):82-91. Review. PMID: 11285167
  166. Clostridium difficile--Associated diarrhea: A review. Mylonakis E, Ryan ET, Calderwood SB. Arch Intern Med. 2001 Feb 26;161(4):525-33. Review.PMID: 11252111 Free article
  167. Cholera vaccines. Ryan ET, Calderwood SB. Clin Infect Dis. 2000 Aug;31(2):561-5. Epub 2000 Sep 7. Review.PMID: 10987721
  168. Health advice and immunizations for travelers. Ryan ET, Kain KC. N Engl J Med. 2000 Jun 8;342(23):1716-25. Review. PMID: 10841875
  169. In vitro and in vivo analyses of constitutive and in vivo-induced promoters in attenuated vaccine and vector strains of Vibrio cholerae. John M, Crean TI, Calderwood SB, Ryan ET. Infect Immun. 2000 Mar;68(3):1171-5.PMID: 10678922 Free article
  170. Optimizing the germfree mouse model for in vivo evaluation of oral Vibrio cholerae vaccine and vector strains. Crean TI, John M, Calderwood SB, Ryan ET. Infect Immun. 2000 Feb;68(2):977-81.PMID: 10639476 Free article
  171. Development of a DeltaglnA balanced lethal plasmid system for expression of heterologous antigens by attenuated vaccine vector strains of Vibrio cholerae. Ryan ET, Crean TI, Kochi SK, John M, Luciano AA, Killeen KP, Klose KE, Calderwood SB. Infect Immun. 2000 Jan;68(1):221-6.PMID: 10603391 Free article
  172. In vivo expression and immunoadjuvancy of a mutant of heat-labile enterotoxin of Escherichia coli in vaccine and vector strains of Vibrio cholerae. Ryan ET, Crean TI, John M, Butterton JR, Clements JD, Calderwood SB. Infect Immun. 1999 Apr;67(4):1694-701.PMID: 10085006 Free article
  173. Oral immunization with attenuated vaccine strains of Vibrio cholerae expressing a dodecapeptide repeat of the serine-rich Entamoeba histolytica protein fused to the cholera toxin B subunit induces systemic and mucosal antiamebic and anti-V. cholerae antibody responses in mice. Ryan ET, Butterton JR, Zhang T, Baker MA, Stanley SL Jr, Calderwood SB. Infect Immun. 1997 Aug;65(8):3118-25.PMID: 9234763 Free article
  174. Protective immunity against Clostridium difficile toxin A induced by oral immunization with a live, attenuated Vibrio cholerae vector strain. Ryan ET, Butterton JR, Smith RN, Carroll PA, Crean TI, Calderwood SB. Infect Immun. 1997 Jul;65(7):2941-9.PMID: 9199470 Free article
  175. Coexpression of the B subunit of Shiga toxin 1 and EaeA from enterohemorrhagic Escherichia coli in Vibrio cholerae vaccine strains. Butterton JR, Ryan ET, Acheson DW, Calderwood SB. Infect Immun. 1997 Jun;65(6):2127-35.PMID: 9169742 Free article
  176. Development of a germfree mouse model of Vibrio cholerae infection. Butterton JR, Ryan ET, Shahin RA, Calderwood SB. Infect Immun. 1996 Oct;64(10):4373-7.PMID: 8926115 Free article
  177. Parasitic infections of the anterior segment. Young JA, Ryan ET. Int Ophthalmol Clin. 1996 Summer;36(3):49-71. Review. PMID: 8989600
  178. Myocardial infarction mimicked by acute cholecystitis. Ryan ET, Pak PH, DeSanctis RW. Ann Intern Med. 1992 Feb 1;116(3):218-20. PMID: 1728205
  179. Hirschsprung's disease: associated abnormalities and demography. Ryan ET, Ecker JL, Christakis NA, Folkman J. J Pediatr Surg. 1992 Jan;27(1):76-81.PMID: 1552451
  180. Polyclonal B-cell lymphocytosis and hypergammaglobulinemia in patients with Gaucher disease. Marti GE, Ryan ET, Papadopoulos NM, Filling-Katz M, Barton N, Fleischer TA, Rick M, Gralnick HR. Am J Hematol. 1988 Dec;29(4):189-94.PMID: 3263798

Reviews, Chapters, and Editorials

  1. Ryan ET. Surgical nosocomial infections.  Resident Staff Phys 1991;2:11-15.
  2. Hibberd PL, Ryan ET, Baker AS.  Pneumocystis carinii, parasitic infections, and the eye.
    In:  Albert DM, Jakobiec FA, eds.  Principles and Practice of Ophthalmology. Philadelphia: WB Saunders; 1994. p. 3065-3077.
  3. Ryan ET, Baker AS.  Systemic bacterial infections and the eye.  In: Albert DM, Jakobiec FA, eds.  Principles and Practice of Ophthalmology.  Philadelphia:  WB Saunders; 1994. p. 3006-3010.
  4. Ryan ET, Swartz MN,  Dermatological infections. In:  Roitt IM, Delves PJ, eds.  Encyclopedia of Immunology, Second Edition.  London:  Academic Press Ltd.; 1998. p. 750-754.
  5. Ryan ET.  Ocular disease. In:  Guerrant RL, Walker DH, Weller PF, eds.  Tropical Infectious Diseases: Principles, Pathogens, and Practice.  Philadelphia  Churchill Livingstone; 1999. p. 1485-1541. (Textbook & pocket manual).
  6. Ryan ET, Maguire JH.  Parasitic infections and the eye. In:  Albert DM, Jakobiec FA, eds.  Principles and Practice of Ophthalmology.  Philadelphia:  WB Saunders; 2000. p. 4917-4939.
  7. Ryan ET. Tropical and travel medicine: literature alert.  Current Infectious Disease Reports 1999:1(5):411-412.
  8. Ryan ET. Tropical and travel medicine: literature alert.  Current Infectious Disease Reports 2000:1(2):3-4.
  9. Ryan ET.  Immunization. In:  Gorroll AH, Mulley Jr AG, eds.  Primary Care Medicine: Office Evaluation and Management of the Adult Patient.  Fourth Edition. Philadelphia:  Lippincott Williams & Wilkins; 2000. 21-36.
  10. Ryan ET. Ocular disease. In: Guerrant RL, Walker DH, Weller PF, eds. Essentials of Tropical Infectious Diseases. Philadelphia: Churchill Livingstone; 2001: 78-87.
  11. Ryan ET, Salam MA. Cholera. In: Katz S, Gershon A, Hotez P, eds. Krugman’s Infectious Diseases of Children, Eleventh Edition.  Philadelphia: Harcourt Health Sciences, 2004;35-46.
  12. Carroll D, Gardner P, Kay BA, Osterholm M, Ryan ET. Transformation of the developing world: socioeconomic matrix. Emerg Infect Dis. 2004;10:2049.
  13. Ryan ET.  Immunization. In:  Gorroll AH, Mulley Jr AG, eds.  Primary Care Medicine: Office Evaluation and Management of the Adult Patient.  Fifth Edition. Philadelphia:  Lippincott Williams & Wilkins, 2005: 27-42.
  14. Chung V, Armstrong EJ, Samuelson J, Ryan ET. Pharmacology of Parasitic Infections. In: Golan DE, Tashjian AH Jr, Armstrong EJ, Galanter JM, Armstrong AW, Arnaout RA, Rose HS, eds.  Principles of Pharmacology: The Pathophysiologic Basis of Drug Therapy. Baltimore:  Lippincott Williams & Wilkins, 2005: 587-602. 
  15. Ryan ET, Durand ML.  Ocular disease. In:  Guerrant RL, Walker DH, Weller PF, eds.  Tropical Infectious Diseases: Principles, Pathogens, and Practice. Second Edition.  New York: Elsevier, 2006: 1554-1600.
  16. Ryan ET, Murray M.  Epidemiology and Biostatistics. In:  Guerrant RL, Walker DH, Weller PF, eds.  Tropical Infectious Diseases: Principles, Pathogens, and Practice. Second Edition.  New York: Elsevier, 2006: 19-25.
  17. Ivers LC, Ryan ET. Pharmacology of Parasitic Infections. In: Golan DE, Tashjian AH Jr, Armstrong EJ, Galanter JM, Armstrong AW, Arnaout RA, Rose HS, eds.  Principles of Pharmacology: The Pathophysiologic Basis of Drug Therapy. Second Edition. Baltimore:  Lippincott Williams & Wilkins, 2007:  631-650. 
  18. Ivers LC, Ryan ET. Protozoal infections of the thorax. In:  Fishman AP, Fishman JA, Grippi MA, Kaiser LB, Senior RM, eds.  Fishman’s Pulmonary Diseases and Disorders. Fourth Edition.  New York: McGraw-Hill, 2008: 2397-2411.
  19. Ryan ET, Durand ML.  Parasitic infections and the eye. In:  Albert DM, Miller JW, eds.  Albert & Jakobiec's: Principles and Practice of Ophthalmology.  Saunders-Elsevier, 3rd Edition, 2008:  4751-4773.
  20. Hill DR, Ryan ET. Diet and education about risks. In:  Ericsson CD, DuPont HL, Steffen R, eds.  Travelers’ diarrhea. 2nd Edition. BC Decker, 2008: 180-190.
  21. Ryan ET. Book Review:  Trachoma- A Blinding Scourge from the Bronze Age to the Twenty-First Century. Hugh R. Taylor. N Engl J Med. 2008; 338:1872-1873.
  22. Larocque RL, Ryan ET.  Immunization. In:  Gorroll AH, Mulley Jr AG, eds.  Primary Care Medicine: Office Evaluation and Management of the Adult Patient.  Sixth Edition. Philadelphia:  Lippincott Williams & Wilkins, 2009: 27-49.
  23. Ghose C, Calderwood SB, Ryan ET. Cholera. In:  Stanberry LR, Barrett ADT, eds. Vaccines for Biodefense and Emerging Infectious Diseases.  Elsevier, 2009: 870-891.
  24. LaRocque EC, Ryan ET. Respiratory Infection. In:  CDC Health Information for International Travel 2010. Elsevier, 2009: 177-181.
  25. Ryan ET, Murray M.  Epidemiology and Biostatistics. In:  Guerrant RL, Walker DH, Weller PF, eds.  Tropical Infectious Diseases: Principles, Pathogens, and Practice. Third Edition.  New York: Elsevier, 2011: 11-16.
  26. Ivers LC, Ryan ET. Pharmacology of Parasitic Infections. In: Golan DE, Tashjian AH Jr, Armstrong EJ, Galanter JM, Armstrong AW, Arnaout RA, Rose HS, eds.  Principles of Pharmacology: The Pathophysiologic Basis of Drug Therapy. Third Edition. Baltimore:  Lippincott Williams & Wilkins, 2012:  629-648.
  27. Ryan ET, Durand ML.  Ocular disease. In:  Guerrant RL, Walker DH, Weller PF, eds.  Tropical Infectious Diseases: Principles, Pathogens, and Practice. Third Edition.  New York: Elsevier, 2011:991-1016.
  28. LaRocque RC, Ryan ET, Calderwood SB.  Acute Infectious Diarrheal Diseases and Bacterial Food Poisoning.  In:  Harrison's Principles of Internal Medicine, 18th Edition.  McGraw Hill, 2012;1084-1090.
  29. Waldor M, Ryan ET.  Cholera and Other Vibrioses.  In:  Harrison's Principles of Internal Medicine, 18th Edition.  McGraw Hill, 2012: 1289-1295.
  30. LaRocque EC, Ryan ET. Respiratory Infection. In:  CDC Health Information for International Travel 2012; Oxford University Press, 2012; 69-71.
  31. Bloom A, Ryan ET. Albendazole. In: Hunter's Tropical Medicine, 9th Edition. Saunders-Elsevier, London, 2013; 1088-90.
  32. Bloom A, Ryan ET. Mebendazole. In: Hunter's Tropical Medicine, 9th Edition. Saunders-Elsevier, London, 2013; 1086-88.
  33. Bloom A, Ryan ET. Dapsone. In: Hunter's Tropical Medicine, 9th Edition. Saunders-Elsevier, London, 2013; 1108-10.
  34. Bloom A, Ryan ET. Pentamidine. In: Hunter's Tropical Medicine, 9th Edition. Saunders-Elsevier, London, 2013; 1104-06.
  35. Bloom A, Ryan ET. Nitazoxanide. In: Hunter's Tropical Medicine, 9th Edition. Saunders-Elsevier, London, 2013; 1106-08.
  36. Ryan ET. Miscellaneous Tissue Protozoa. In: Hunter's Tropical Medicine, 9th Edition. Saunders-Elsevier, London, 2013; 791.
  37. Ryan ET.  The cholera pandemic, still with us after half a century: time to rethink. PLoS Negl Trop Dis 5(1): 2011.  e1003. doi:10.1371/journal.pntd.0001003
  38. LaRocque EC, Ryan ET. Respiratory infection. In:  CDC Health Information for International Travel 2014, Oxford University Press; 2013:  76-78.
  39. Larocque RL, Ryan ET.  Immunization. In:  Gorroll AH (ed).  Primary Care Medicine: Office Evaluation and Management of the Adult Patient.  7th Edition. Philadelphia:  Wolters Klower Health, 2014:  23-40.
  40. Ivers LC, Ryan ET.  Protozoal infections of the thorax. In:  Fishman AP, Fishman JA, Grippi MA, Kaiser LB, Senior RM, eds.  Fishman’s Pulmonary Diseases and Disorders. Fifth Edition. New York, McGraw Hill. (in press).
  41. Waldor MK, Ryan ET.  Cholera.  Principals and Practice Infectious Diseases; Mandell, Douglas, Bennett, Eds. Bennett JE, Dolin R, Blaser MJ, 8th Edition; Saunders-Elsevier (in press). 2014.  2471-2479.
  42. Harris JB, Ryan ET.  Enteric fevers and other causes of fever and abdominal symptoms.  Principals and Practice Infectious Diseases; Mandell, Douglas, Bennett, Eds. Bennett JE, Dolin R, Blaser MJ, 8th Edition; Saunders-Elsevier (in press). 2014.  1270-1282.
  43. Ryan ET. ASTMH Presidential Address: Eyes on the prize: lessons from the cholera wars for modern scientists, physicians and public health officials. Am. J. Trop. Med. Hyg., 89(4), 2013, pp. 610–614.
  44. Ryan ET. The intestinal pathobiome: its reality and consequences among infants and young children in resource-limited settings. J Infect Dis. 2013 Dec;208(11):1732-3. 
  45. LaRocque RC, Ryan ET, Calderwood SB.  Acute infectious diarrheal diseases and bacterial food poisoning.  In:  Harrison's Principles of Internal Medicine, 19th Edition.  McGraw Hill (in press).
  46. Keusch GT, Rosenberg IH, Denno DM, Duggan C, Guerrant RL, Lavery JV, Tarr PI, Ward HD, Black RE, Nataro JP, Ryan ET, Bhutta ZA, Coovadia H, Lima A, Ramakrishna B, Zaidi AKM, Burgess DCH, Brewer T. Implications of acquired environmental enteric dysfunction for growth and stunting in infants and children living in low- and middle-income countries. Food and Nutrition Bulletin 2013; 34 (3). 357-364.
  47. LaRocque RC, Ryan ET. Respiratory infections. In: Zuckerman J,  Brunette G, Leggat P (eds), Essential Travel Medicine, Wiley-Blackwell (in press).
  48. Waldor M, Ryan ET.  Cholera and Other Vibrioses.  In:  Harrison's Principles of Internal Medicine, 19th Edition.  McGraw Hill, (in press).
  49. Blazes DL, Riddle MS, Ryan ET. The local importance of global infectious diseases. Trop Dis Trav Med Vaccines. 2015; 1:5.
  50. LaRocque EC, Ryan ET. Respiratory infection. In:  CDC Health Information for International Travel 2016, Oxford University Press, 2016:  78-80.
  51. Ryan ET. Cost analysis of travel medicine. In:  CDC Health Information for International Travel 2016, Oxford University Press 2016:  40-41.
  52. Ivers LC, Ryan ET. Pharmacology of Parasitic Infections. In: Golan DE, Tashjian AH Jr, Armstrong EJ, Galanter JM, Armstrong AW, Arnaout RA, Rose HS, eds.  Principles of Pharmacology: The Pathophysiologic Basis of Drug Therapy. 4th Edition. Baltimore:  Lippincott Williams & Wilkins (in press).
  53. Ryan ET. Antiparasitic Agents.  In: Long SS, Prober CG, Fisher M, eds.  Principle and Practice of Pediatric Infectious Diseases.  5th Edition. Elsevier (in press).
  54. Ryan ET. Troubling news out of Asia on typhoid:  a coming storm. Lancet Infectious Diseases (in press).
  55. Emily P. Hyle, Ryan ET. Cost analysis of travel medicine. In: CDC Health Information for International Travel 2018, Oxford University Press, (in press).
  56. Saleri N, Ryan ET. Respiratory Infections. In: Keystone JS, Freedman DO, Kozarsky PE, Connor BA, Nothdurft HD. Travel Medicine, 4th Edition. Elsevier-Saunders (in press).
  57. LaRocque EC, Ryan ET. Respiratory infection. In: CDC Health Information for International Travel 2018, Oxford University Press, (in press).
  58. Waldor M, Ryan ET. Cholera and Other Vibrioses. In: Harrison's Principles of Internal Medicine, 20th Edition. New York. McGraw Hill (in press).

Textbooks

  1. Hunters's Tropical Medicine and Emerging Infectious Diseases, Editors: A Magill, ET Ryan, D Hill, T Solomon. 9th Edition, Saunders-Elsevier, London, 2013 (ISBN: 978-1-4160-4390-4).
  2. Hunter's Tropical Medicine and Emerging Infectious Diseases, Editors: ET Ryan (lead editor), N Aronson, T Endy, D Hill, T Solomon. 10th Edition, Saunders-Elsevier, London, (in process).

Clinical Communications/Case Reports

  1. Moore AC, Ryan ET, Waldron MA.  Case records of the Massachusetts General Hospital. Weekly clinicopathological exercises. Case 20-2002. A 37-year-old man with fever, hepatosplenomegaly, and a cutaneous foot lesion after a trip to Africa. N Engl J Med. 2002;346:2069-76.
  2. Brainard DM, Ryan ET.  Images in clinical medicine. Thoracic echinococcosis. N Engl J Med. 2003;348:528.
  3. Kao AY, Munandar R, Ferrara SL, Systrom DM, Sheridan RL, Cash SS, Ryan ET.  Case records of the Massachusetts General Hospital. Case 19-2005. A 17-year-old girl with respiratory distress and hemiparesis after surviving a tsunami. N Engl J Med. 2005; 352: 2628-2636.
  4. Ryan ET, Felsenstein D, Aquino SL, Branda JA, Morgan JG.  Case records of the Massachusetts General Hospital. Case 39-2005. A 63-year-old woman with a positive serologic test for syphilis and persistent eosinophilia. N Engl J Med 2005; 353:2697-2705.
  5. Ryan ET, Aquino SL, Kradin RL.  Case records of the Massachusetts General Hospital. Weekly clinicopathological exercises. Case 29-2007.  A 51-year old man with gastric cancer and lung nodules. N Engl J Med. 2007; 357: 61-68.
  6. Ryan ET, Madoff LC, Ferraro MJ.  Case records of the Massachusetts General Hospital. Case 20-2011. A 30-year-old man with diarrhea after a trip to the Dominican Republic.  N Engl J Med. 2011 Jun 30;364(26):2536-41. PMID: 21714651
  7. Ryan ET, Cronin CG, Branda J.  Case records of the Massachusetts General Hospital. Weekly clinicopathological exercises. Case -2010.  A 34-year old man with paralysis and diarrhea. N Engl J Med. 2011 Dec 15;365(24):2306-16.
  8. Kitchens WH, Liu C, Ryan ET, Fernandez-Del Castillo C. Hepatic hydatid cyst: a rare cause of recurrent pancreatitis.  J Gastrointest Surg. 2014 Aug 23.
  9. Lammert S, Ryan ET, LaRoque RC. JAMA PATIENT PAGE. Safe and Healthy International Travel. JAMA. 2015 Dec 15;314(23):2580.

Contact

Contact Us

Edward T. Ryan, MD

Massachusetts General Hospital

Jackson 50455 Fruit Street Boston, MA 02114
  • Phone: 617-726-6175
  • Fax: 617-726-7416
  • Email Us

Lucia Pizzo Flaherty
Administrative Coordinator
Email

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