The Vincent Center for Reproductive Biology

Hideo Sakamoto, Ph.D.

Research Fellow, Vincent Center for Reproductive Biology at Massachusetts General Hospital, Harvard Medical School

Education and Training
Fujita Health University School of Health Sciences (Japan), BS, Medical Technology
Fujita Health University Graduate School of Medicine (Japan), PhD, Biochemistry

Positions and Honors
Positions and Employment
1987-1990: Medical Technologist, Department of Joint Research Laboratory of Clinical Medicine Fujita Health Univ. Hospital
1990-1998: Research Assistant, Division of Molecular Biology, Institute for Comprehensive Medical Science, Fujita Health Univ. School of Medicine
1998-2000: Research Fellow, Department of Pathology, Massachusetts General Hospital, Harvard Medical School
2000-2003: Instructor, Dept of Biochemistry, Fujita Health University School of Medicine
2002-2003: Visiting Assistant Professor, Aichi University of Education
2003-present: Research Fellow, Massachusetts General Hospital

Professional Memberships
1995-present: Japanese Biochemical Society
1996-present: Japanese Association of Medical Technologists
1997-present: American Association for Clinical Chemistry
1998-present: National Academy of Clinical Biochemistry
2001-present: Japanese Society of Laboratory Medicine
2000-present: Japanese Society of Clinical Chemistry (Intrntnl committee)
2002-present: Japanese Society for Clinical Laboratory Automation (POCT)

Honors and Awards
2000: Student Stipend from American Association for Clinical Chemistry
2002: Grant in Aid for Scientific Research

Selected peer-reviewed publications (in chronological order)

1) Sakamoto, H., Fujita, K., Ikegame, M., and H., K. Partialpurification of human liver GTP cyclohyrolase I. Bulletin of the Japanese society for Neurochemistry, 34: 222-223, 1995.

2) Sakamoto, H., Fujita, K., Ikegame, M.,and Kuzuya, H. Different complex forms of human liver GTPCyclohydrolase I. Biogenic Amines, 12: 55-67, 1996.

3) Kuzuya, H., Fujita, K., Sakamoto, H., Kawai, K., Shimpo, K., Beppu, H., and Ito, T. Simultaneous measurement ofmonoamines,their metabolites and precursor amino acids in plasma and urine from healthy subjects by HPLC/ multi-electrode electrochemistry.Biogenic Amines, 12: 9-18, 1996.

4) Sakamoto, H., Fujita, K., Taradaira, R., andH., K.Comparative study for animal GTP Cyclohydrolase I. Bulletin of the Fujita Medical Society, 20: 153-157, 1996.

5) Sakamoto, H., Fujita, K., Ikegame, M., and H., K. The effect of catecholamine metabolism inhibitors for GTP Cyclohydrolase I activity in rat brain. Bulletin of the Japanese society for Neurochemistry, 35: 638-639, 1996.

6) Sakamoto, H., Fujita, K., Ikegame, M., and Kuzuya, H. Influence of an aromatic amino acid decarboxylase inhibitor on GTP cyclohydrolase I activity in the rat brain. Pteridines, 8: 206-210, 1997.

7) Kuzuya, H., Fujita, K., and Sakamoto, H. Influences of catecholamine contents on tetrahydrobiopterin metabolism.Ptridines, 12: 39-43, 1997.

8) Itoh, M., Uchimura, K., Hayakawa,N., Makino, M., Hayashi,R., Nagata, M., Kakizawa, H., Nagasaka, A., Sakamoto, H., and Kuzuya, H. Surface expressionand releaseof soluble forms of CD8 and CD23in CD40- and IL-4-activated mononuclearcells from patients with Graves' disease (GD). Clinical & Experimental Immunology., 113: 309-314, 1998.

9) Sakamoto, H., Fujita,K., andH., K. Relationships between biosynthesis of catecholamine and tetrahydrobiopterin in PC12 cells. Bulletin of the Fujita medical society, 21: 283-286, 1998.

10) Sakamoto, H., Kuzuya, H., Tamaru,M., Sugimoto, S., Shimizu, J., Fukushima, M., Yazaki, T., Yamazaki,T., and Nagata, Y. Developmental changes in the NGF content inthe brain of young, growing, low-birth-weight rats. Neurochemical Research., 23: 115-120, 1998.

11) Mano, T., Sakamoto, H., Fujita, K., Makino, M., Kakizawa,H., Nagata, M., Kotake, M., Hamada, M., Uchimura, K., Hayakawa, N.,Hayashi, R., Nakai, A., Itoh, M., Kuzuya, H., andNagasaka, A. Effects of thyroid hormone on catecholamine and its metabolite concentrations in rat cardiac muscle and cerebral cortex. Thyroid., 8: 353-358, 1998.

12) Tager, A. M., Luster, A. D.,Leary, C. P., Sakamoto, H., Zhao, L. H., Preffer, F., and Kradin, R. L. Accessory cells with immunophenotypic and functional features of monocyte-derived dendritic cells are recruited to the lung during pulmonary inflammation. Journal of Leukocyte Biology., 66: 901-908, 1999.

13) Kradin, R. L., Sakamoto, H., Preffer, F. I., Dombkowski, D., Springer, K. M., and Leary, C. P. Accumulation of macrophages with dendritic cell characteristics in the pulmonary response to Listeria. American Journal of Respiratory & Critical Care Medicine., 161: 535-542, 2000.

14) Kradin, R. L., Sakamoto, H.,Zhao, L. H., and Leary, C. P. Bleomycin lung injury in interleukin-12 knockout mice. Chest., 120: S10-11., 2001.

15) Harada, N., Matsumoto, T., Yoshimura, N., Sakamoto, H., and Honda, S. Analysis of transcriptional regulation of human breast aromatase by in vitro and in vivo studies. Journal ofSteroid Biochemistry & Molecular Biology., 79: 151-156, 2001.

16) Sakamoto, H., Zhao, L. H., Jain, F., and Kradin, R. IL-12p40(-/-) mice treated with intratracheal bleomycin exhibit decreased pulmonary inflammation and increased fibrosis. Experimental & Molecular Pathology., 72: 1-9, 2002.