A new study finds that Interleukin-3 (IL-3), an inflammatory factor most associated with allergic reactions, appears to have an important role in the overwhelming, life-threatening immune reaction called sepsis. In the March 13 issue of Science, investigators from Massachusetts General Hospital (MGH) describe finding that the presence of IL-3 is essential to the development of sepsis in a mouse model of the condition and that IL-3 levels in human patients with sepsis are higher in those at greater risk of dying.
“Sepsis is an extremely dangerous conditions that claims up to half a million lives in the U.S. every year and millions worldwide” says Filip Swirski, PhD, of the MGH Center for Systems Biology, senior author of the study. “Our study shows that, in response to infection, IL-3 promotes the production of inflammatory monocytes and neutrophils – immune cells that are sources of the so-called ‘cytokine storm’ that underlies sepsis.”
Sepsis occurs when the immune system overreacts to an infection or injury, producing excessive levels of signaling molecules called cytokines that attract immune cells. Elevated levels of those cells secrete more cytokines, and this cytokine storm recruits even more immune cells, fuelling a vicious cycle. Instead of stopping the initial infection, immune factors attack the body’s tissues and organs, causing organ failure and death. Many aspects of the mechanism behind sepsis are poorly understood, and since IL-3 is known to contribute to the production and proliferation of several types of white blood cells – including those that produce factors involved in the cytokine storm – Swirski’s team investigated its potential involvement in sepsis.
First they showed in a mouse model of the condition that the absence of IL-3 both prevented the development of sepsis and reduced the production of sepsis-associated immune cells and cytokines. They also found that injecting IL-3 into mice in which expression of the factor had been knocked out restored their susceptibility to sepsis and that blocking the interaction between IL-3 and its receptor on immune cells reduced the production of sepsis-associated factors.
A search for the source of sepsis-inducing IL-3 revealed that it was produced by B cells in the spleen, thymus and lymph nodes – cells that proved to be the IRA B cells first identified by Swirski’s team in a 2012 Science paper. Those cells also produce the growth factor GM-CSF; and without IRA B cell-derived GM-CSF, animals in which sepsis was induced died faster and in greater numbers than did control animals. But while GM-CSF protected against sepsis in the earlier study, IL-3 generated by the same cell population increased production of inflammatory cytokines that aggravate sepsis in the current investigation – an observation that reflects the delicate balance of immune reactions required to defend against infection without inflicting the damage that leads to sepsis.
To confirm that the results observed in their mouse model reflect what could happen in human patients, the researchers analyzed blood samples from 60 sepsis patients involved in a previous study and found that IL-3 levels during the first 24 hours after the onset of sepsis were higher in patients that eventually died. The investigators prospectively measured levels of IL-3 and monocytes in another group of 37 patients being treated for sepsis, finding that the onset of sepsis was accompanied by a rapid increase in cytokine levels and that patients with the highest IL-3 levels were most likely to die.
“Questions have been raised about how well mouse models of sepsis mirror the human disease, but our study shows that the role of IL-3 we discovered in mice is also important in human patients,” says Swirski, who is an associate professor of Radiology at Harvard Medical School. “Since even seemingly healthy people have some level of IL-3 in their blood, it will be important to investigate whether those with higher levels are at greater risk of sepsis and other complications of infection and whether targeting IL-3 and the pathways it controls could be a treatment for sepsis. Overall we need a better understanding of what IL-3 does in sepsis and in other infectious and non-infectious diseases. We currently are investigating whether IL-3 is involved in what is called the suppressive phase of sepsis, which can follow the initial inflammatory phase and put patients at risk of developing secondary infections.”
Co-lead authors of the Science paper are Georg F. Weber, MD, PhD, also co-corresponding author, Benjamin Chousterman, MD, and Shun He, PhD, all of the MGH Center for Systems Biology. Additional co-authors are Ashley M. Fenn, Manfred Nairz, Atsushi Anzai, Yoshiko Iwamoto, Clinton S. Robbins, Lorette Noiret, Matthias Nahrendorf, and Ralph Weissleder, MGH Center for Systems Biology; Thorsten Brenner, Florian Uhle, Stefan Hofer, and Markus A. Weigand, University of Heidelberg; and Sara L. Maier, Tina Zonnchen, Nuh N. Rahbari, Sebastian Scholch, Anne Klotzsche-von Ameln, Triantafyllow Chavakis, and Jurgen Weitz, Technische Universitat Dresden. Support for the study includes National Institutes of Health grants HL095612 and AI104695 and grants from the German Research Foundation, Société Française d’Anesthésie-Réanimation, Institut Servier, Fondation Groupe Pasteur Mutualité, and the Fulbright Program.
Massachusetts General Hospital (www.massgeneral.org), founded in 1811, is the original and largest teaching hospital of Harvard Medical School. The MGH conducts the largest hospital-based research program in the United States, with an annual research budget of more than $760 million and major research centers in HIV/AIDS, cardiovascular research, cancer, computational and integrative biology, cutaneous biology, human genetics, medical imaging, neurodegenerative disorders, regenerative medicine, reproductive biology, systems biology, transplantation biology and photomedicine.
Sue McGreevey, firstname.lastname@example.org, 617 724-2764