Explore This Core

Overview

The Genetic Animal Models Core provides emerging genetic technology and model systems that enable transformative investigation into the impact of human genetic variants associated with IBD, including infantile and early-onset IBD.

The Core provides services for investigations of in vivo function of IBD-associated genetic variants with the goals of identifying relevant pathways, defining microbiome-host interactions, gaining insight into systems that control intestinal inflammation, and understanding mechanisms of recovery from epithelial injury.

To complement in vivo studies, technical support relevant to in vitro techniques such as organoid and primary cell models are available. The Core facilitates access to a bank of genotyped mouse- and human-derived organoids as well as optimized tools, technologies, and protocols to manipulate organoids and primary epithelial cells. These techniques include generating spheroids or monolayers, CRISPR-mediated genetic engineering, culturing at the air-liquid interface, co-culturing with myeloid or T cells, and transplanting organoids into recipient mice. 

Through project-focused consultations, educational programs including extensive training opportunities, open lab meetings and workshops, and direct experimental support (e.g., providing protocols and novel model systems), the Core serves a key role in facilitating access to state-of-the-art genome editing approaches and experimental systems for research programs that focus on digestive diseases.

Core Personnel

Cornelius Terhorst, PhD
Co-Director

Hans-Christian Reinecker, MD
Co-Director

Kate Jeffrey, PhD
Associate Director

Omar Yilmaz, MD, PhD
Associate Director

Atul Bhan, MD
Consultant

Thorsten Mempel, MD, PhD
Consultant


Objectives

  1. Support investigations in mice aimed at elucidating the contribution of IBD-related genetic variants and related genes to the pathogenesis of IBD in humans
  2. Functional in vitro and in vivo imaging of cellular responses and migration in the mucosal and peripheral immune system
  3. Support sharing of model systems and tools to foster collaborations among CSIBD investigators

Services

  • Genome editing in ES cells
  • Tissue- and cell type-specific gene targeting
  • Model systems for IBD
  • Functional imaging
  • Mouse models of intestinal inflammation
  • Models of host defense
  • Access to a bank of genotyped mouse- and human-derived intestinal organoids as well as tools, technologies, and protocols for manipulation of these systems