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We aim to understand how GPCR signaling is integrated with other signaling pathways to generate bioactive signaling lipids, the leukotrienes (LTs) in myeloid cells. These studies combine biochemistry and molecular imaging to study novel complexes that are assembled on the nuclear envelope to initiate LT synthesis. Because they are transiently assembled complexes held together by weak interactions, we combine multiple biochemical and molecular imaging approaches that include Fluorescence Lifetime Imaging Microscopy (FLIM) and super resolution microscopy (STORM and PALM). We have established collaborations within and outside of Massachusetts General Hospital in the field of super resolution microscopy and how macromolecular complexes are organized. We are also in collaboration with the Vasculitis and Glomerulonephritis Center at Mass General where we are studying the organization of the leukotriene synthetic complex and the role of RGS proteins in patients with AAV.