Dou

The Dou laboratory studies the biology of aging and aims to develop new strategies to suppress aging and age-associated diseases.

Overview

Our laboratory is interested in the mechanisms of mammalian aging. Aging is a chronic process associated with deteriorating physiological functions and increasing risks for multiple diseases, such as cancer, cardiovascular diseases, neurodegeneration, and metabolic disorders. Our goals are to develop new approaches to facilitate tissue regeneration and to intervene in diseases of aging.

Research topics in the laboratory include autophagy, chromatin and epigenetics, immunology, and metabolism, in the broad scenarios of aging and cancer.

Lab Members

Zhixun Dou, PhD

Research Projects

(1) Nuclear degeneration in aging and cancer

The nucleus is a central cellular entity. Our study suggests that the nucleus undergoes profound degeneration during aging and tumorigenesis. Nuclear components, including nuclear lamina and chromatin, can be degraded by autophagy (Dou et al, Nature, 2015). While autophagy is generally viewed as a cytoplasmic recycling mechanism, the degradation of nuclear materials is beginning to unravel. We are particularly interested in asking:

  • What are nuclear and chromatin substrates of autophagy?
  • How is nuclear autophagy regulated?
  • How does nuclear autophagy impact chromatin and the epigenome?
  • What are the functional roles of nuclear degeneration in aging and cancer? 

(2) The biology of cellular senescence 

Cellular senescence, also referred to as the Hayflick limit, is a stable form of cell cycle arrest. Senescence restricts proliferation of damaged cells, and hence is a tumor suppressive mechanism. However, senescent cells accumulate in aged tissues, contributing to many age-associated diseases. Our study shows that senescent cells generate chromatin fragment in the cytoplasm, which is interpreted by the cell as a “danger signal” and activates the cytosolic DNA sensing cGAS-STING pathway, leading to inflammation (Dou et al, Nature, 2017). The pro-inflammatory feature of senescence triggers immuno-surveillance of oncogenic cells, but contributes to age-associated diseases. We aim to address several fundamental questions of senescence:

  • How is cytoplasmic chromatin generated and regulated?
  • What is the interaction between senescent cells and the immune system?
  • How can we target this pro-inflammatory pathway to treat diseases?

(3) Mechanisms of mammalian aging

We are broadly interested in the biology of aging at the molecular, cellular, and organismal levels. The laboratory employs multiple mouse models to study alterations of metabolism and the immune system during aging. Our long-term goal is to develop novel approaches to treat age-related diseases and to promote “healthy aging without diseases”.

Research Positions

 Dou lab is currently accepting CVs for the following positions:

  • Postdoctoral fellows: 

Postdoctoral fellow positions are available in the Dou lab, to investigate cutting-edge research topics on aging, cancer, cell biology, and epigenetics.

MGH and Harvard offer unique opportunities for scientific growth and interactions in a highly collaborative and dynamic environment. Candidates are expected to demonstrate a track record of research achievement and have excellent communication skills. Please contact Dou with a cover letter, CV, and contact information for three letters of recommendation.

  • Research technicians:

We are looking for highly motivated individuals to join our lab. Successful candidates will work directly with scientists on projects investigating aging and cancer, and will be exposed to sophisticated experimental approaches including viral genetics, advanced imaging, and mouse models of diseases.

Two or more years’ commitment required (special occasions will be considered). Previous research experiences in molecular and cellular biology strongly preferred. Mouse experience preferred but not required. Please contact Dou with a cover letter, CV, and contact information for three letters of recommendation.

Publications

Full list of publications:

https://www.ncbi.nlm.nih.gov/pubmed/?term=zhixun+dou

 

Selective publications:

 

Dou Z*, Berger SL* (co-corresponding authors). Senescence Elicits Stemness: A Surprising Mechanism for Cancer Relapse. Cell Metab. 2018 Apr 3;27(4):710-711.

 

Dou Z, Ghosh K, Vizioli MG, Zhu J, Sen P, Wangensteen KJ, Simithy J, Lan Y, Lin Y,

Zhou Z, Capell BC, Xu C, Xu M, Kieckhaefer JE, Jiang T, Shoshkes-Carmel M, Tanim

KM, Barber G, Seykora JT, Millar SE, Kaestner KH, Garcia BA, Adams PD, Berger SL.

Cytoplasmic chromatin triggers inflammation in senescence and cancer. Nature. 2017 Oct 19;550(7676):402-406.

 

Dou Z, Xu C, Donahue G, Shimi T, Pan JA, Zhu J, Ivanov A, Capell BC, Drake AM,

Shah PP, Catanzaro JM, Ricketts MD, Lamark T, Adam SA, Marmorstein R, Zong WX,

Johansen T, Goldman RD, Adams PD, Berger SL. Autophagy mediates degradation of

nuclear lamina. Nature. 2015 Nov 5;527(7576):105-9.

 

Zhu J, Sammons MA, Donahue G, Dou Z, Vedadi M, Getlik M, Barsyte-Lovejoy D, Al-

Awar R, Katona BW, Shilatifard A, Huang J, Hua X, Arrowsmith CH, Berger SL. Gain-of-function

p53 mutants co-opt chromatin pathways to drive cancer growth. Nature. 2015

Sep 10;525(7568):206-11.

 

Dou Z, Pan JA, Dbouk HA, Ballou LM, Deleon JL, Fan Y, Chen JS, Liang Z, Li G,

Backer JM, Lin RZ, Zong WX. Class IA PI3K p110β subunit promotes autophagy

through Rab5 small GTPase in response to growth factor limitation. Molecular Cell.

2013 Apr 11;50(1):29-42.

 

Jaber N, Dou Z, Chen JS, Catanzaro JM, Jiang YP, Ballou LM, Selinger E, Ouyang X,

Lin RZ, Zhang J, Zong WX. The Class III PI3K Vps34 plays an essential role in

autophagy and in the heart and liver function. PNAS. 2012 Feb 7;109(6):2003-8.

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