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Research at Mass General
AAV Request Service
AAV vectors made available through the core carry AAV2 ITR elements serotyped with either AAV1, 2, 4, 5, 8, 9, or rh10 capsids. AAVs are purified by density gradient centrifugation, followed by FPLC chromatography. Featuring a number of fluorescent proteins, secreted and cytosolic luciferases, bicistronic constructs and tissue-specific promoters, our AAVs are useful for infecting cell cultures as well as tissues in living organisms.
Lentivirus Request new service
Lentiviral vectors made available through the Core are cary HIV-1 LTRs, pseudotyped with the VSV-G envelope protein.
Plasmid Prep Request Service
In order to achieve the highest viral titer possible, the MGH Vector Core requires sterile, endotoxin-free plasmid DNA from its customers. While commercial DNA prep kits are available, many labs find it more convenient and cost-effective to have us do this for them. Below is a table showing the cost for our Plasmid Preparation.
Plasmid Sharing Request Service
Retrovirus Request Service
Virus Aliquots Request Service
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