Wednesday, May 10, 2017

Molecular imaging reveals mechanism for resistance to immune checkpoint blockade

Macrophages that rapidly remove drugs from T cells may be new treatment target

Among today’s most promising weapons against cancer is the use of therapies that direct the immune system against a tumor. One approach – immune checkpoint blockade – is designed to circumvent the “off switches” that prevent the immune system from attacking healthy tissues but also can shield a tumor from the immune response. These drugs have had remarkable success in some but not all patients, and long-term survival has been achieved in a minority of patients.

Now a study from Massachusetts General Hospital (MGH) investigators has identified a surprising mechanism for resistance to immune checkpoint blockade. In their paper published online in Science Translational Medicine, the researchers describe finding that an antibody-based drug designed to block the immunosuppressive molecule PD-1 is removed from its target T cells by macrophages within minutes of administration in several mouse models of cancer. They also identify the molecular mechanism behind this drug capture, which could lead to ways to prevent the process.

“Immune checkpoint blockers are very effective in some patients but not others, and our current ability to understand why treatments work or fail is quite limited,” says Mikael Pittet, PhD, director of the Cancer Immunology Program in the MGH Center for Systems Biology and senior author of the report. “Using high-resolution molecular imaging to track immune checkpoint drugs in real time, we were able to discover what was happening, devise ways to extend the time the drug binds to its target and improve treatment efficacy in our models.”

Immune checkpoint molecules like PD-1 are expressed on the surface of CD8 T cells – the immune system’s “killer cells” that attack cells that are damaged or diseased, including cancer cells – and act to suppress an inappropriate T cell response. Monoclonal antibodies that block pathways controlled by checkpoint molecules are the basis of current checkpoint blockade drugs. The MGH team used intravital microscopy – which examines biological processes in living animals through tiny implanted windows – to track the activity of an antiPD-1 drug in mouse models of colon cancer.

As expected, the labeled antibody was observed to bind to PD-1 molecules on CD8 T cells within a few minutes. But as little as 20 minutes later, the drug had been taken up by macrophages within the tumors. The same process of rapid antibody binding to PD-1 molecules on CD8 T cells, followed by macrophage uptake, was observed in models of melanoma and lung cancer. To determine how the antibodies were being removed from T cells, the researchers first confirmed that the macrophages neither expressed PD-1 molecules nor did they take up antibody not bound to T cells.

A macrophage (red) removing PD-1-blocking antibodies (yellow) from the surfaces of neighboring CD8 T cells (blue), cutting short the drug’s activity. (Sean Arlauckas, PhD, Christopher Garris, Mikael Pittet, PhD, and Ralph Weissleder, MD, PhD, Center for Systems Biology, Massachusetts General Hospital.)


Experiments in mouse and human tumor cells revealed that antibody removal was accomplished through the interaction of the Fc region – the portion of an antibody that communicates with and directs the action of immune cells – and Fc receptors on the surface of macrophages. Administering an Fc receptor inhibitor prior to anti-PD-1 treatment both extended the binding of the drug to CD8 T cells and led to complete tumor disappearance in a mouse model. Whether a similar strategy could improve the results of immune checkpoint blockade in human patients may be answered by current clinical trials that combine immune checkpoint blockers with drugs targeting macrophages, which have number of detrimental effects in cancer.

“Our observations would not have been possible without a method of dynamically imaging drug action on a cellular level,” says Pittet, who is an associate professor of Radiology at Harvard Medical School. “Our platform for imaging anti-PD-1 in live animals can easily be adapted to study additional checkpoint blockade agents, so we are building a program to track the cellular interactions that will allow us to decipher drug mechanisms and hopefully leverage knowledge into engineering better therapeutics.”

The lead authors of the Science Translational Medicine report are Sean Arlauckas, PhD, and Christopher S. Garris, MGH Center for Systems Biology. Additional co-authors are Rainer Kohler, PhD, Michael Cuccarese, PhD, Katherine Yang, PhD, Miles Miller, PhD, Jonathan Carlson, MD, PhD, and Ralph Weissleder, MD, PhD, Center for Systems Biology; Maya Kitaoka and Robert Anthony, PhD, MGH Center for Immunology and Infectious Disease; and Gordon Freeman, PhD, Dana-Farber Cancer Institute. Support for the study includes the Samana Cay MGH Research Scholar Fund, National Institutes of Health grants R01AI084880, R01CA164448, R21CA190344, U54-CA151884, P50CA086355, DP2AR068272-01, and HL084312; Department of Defense grant PC140318, and the David H. Koch–Prostate Cancer Foundation Award in Nanotherapeutics.

Massachusetts General Hospital, founded in 1811, is the original and largest teaching hospital of Harvard Medical School. The MGH Research Institute conducts the largest hospital-based research program in the nation, with an annual research budget of more than $800 million and major research centers in HIV/AIDS, cardiovascular research, cancer, computational and integrative biology, cutaneous biology, genomic medicine, medical imaging, neurodegenerative disorders, regenerative medicine, reproductive biology, systems biology, photomedicine and transplantation biology. The MGH topped the 2015 Nature Index list of health care organizations publishing in leading scientific journals and earned the prestigious 2015 Foster G. McGaw Prize for Excellence in Community Service. In August 2016 the MGH was once again named to the Honor Roll in the U.S. News & World Report list of "America’s Best Hospitals."

Media contact: Katie Marquedant, kmarquedant@partners.org, 617 726-0337

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