Massachusetts General Hospital, Nephrology Division
149 13th Street
Boston,
MA
02129
Email: rmorizane@mgh.harvard.edu
The Morizane Lab focuses on kidney disease studies using stem cell-derived tissues. Specifically, we are generating 3D kidney tissues termed organoids from human pluripotent stem cells (hPSCs). Kidney organoids consist of multiple cell types of kidneys, serving as a novel tool for drug development and regenerative medicine. Our group is divided into three main subgroups:
Ryuji Morizane, MD, PhD
Principal Investigator
Research Staff, Nephrology Division, Massachusetts General Hospital
Assistant Professor, Department of Medicine, Harvard Medical School
Affiliated Faculty, Harvard Stem Cell Institute
Visiting Scholar, Wyss Institute, Harvard University
Dr. Morizane is a physician-scientist at Massachusetts General Hospital and Harvard Medical School. He has a long-standing interest in translational research using hPSCs for kidney diseases and drug discovery. He has been working on pluripotent stem cell research since 2007 and established protocols to induce kidney lineage cells. His seminal work enabled the generation of nephron progenitor cells and kidney organoids from hPSCs with high efficiency, leading to his NIH Director’s New Innovator Award in 2019.
Navin Gupta
Instructor
Ken Hiratsuka
Postdoctoral fellow
Kazumi Ida
Research Technician
Kenichi Kobayashi
Postdoctoral fellow
Astia Safitri
Postdoctoral fellow
Eleanor Wu
High school student
Chengcheng Zhang
Reseach Technician
We accept fellows and visiting scientists from both academia and industry. Innovation Fellows Program enables personnel exchanges between our lab and participating industry. Should you have any questions, please contact Dr. Morizane.
Ryuji Morizane, MD, PhD
Email: rmorizane@mgh.harvard.edu
Traitteur Tamara, Chengcheng Zhang, and Ryuji Morizane. 1/9/2022. “iPSCs - State of the Science.” In Elsevier. Publisher's Version
Navin Gupta, Takuya Matsumoto, Ken Hiratsuka, Edgar Garcia Saiz, Pierre Galichon, Tomoya Miyoshi, Koichiro Susa, Narihito Tatsumoto, Michifumi Yamashita, and Ryuji Morizane. 2022. “Modeling injury and repair in kidney organoids reveals that homologous recombination governs tubular intrinsic repair.” Sci Transl Med, 14, 634, Pp. eabj4772. Publisher's Version Abstract
Astia Rizki-Safitri, Tamara Traitteur, and Ryuji Morizane. 2021. “Bioengineered Kidney Models: Methods and Functional Assessments.” Function, 2, 4. Publisher's Version Abstract
Navin Gupta and Ryuji Morizane. 2021. “Kidney development to kidney organoids and back again.” Semin Cell Dev Biol. Abstract
Krishanu Saha, Erik J Sontheimer, PJ Brooks, Melinda R Dwinell, Charles A Gersbach, David R Liu, Stephen A Murray, Shengdar Q Tsai, Ross C Wilson, Daniel G Anderson, Aravind Asokan, Jillian F Banfield, Krystof S Bankiewicz, Gang Bao, Jeff WM Bulte, Nenad Bursac, Jarryd M Campbell, Daniel F Carlson, Elliot L Chaikof, Zheng-Yi Chen, Holland R Cheng, Karl J Clark, David T Curiel, James E Dahlman, Benjamin E Deverman, Mary E Dickinson, Jennifer A Doudna, Stephen C Ekker, Marina E Emborg, Guoping Feng, Benjamin S Freedman, David M Gamm, Guangping Gao, Ionita C Ghiran, Peter M Glazer, Shaoqin Gong, Jason D Heaney, Jon D Hennebold, John T Hinson, Anastasia Khvorova, Samira Kiani, William R Lagor, Kit S Lam, Kam W Leong, Jon E Levine, Jennifer A Lewis, Cathleen M Lutz, Danith H Ly, Samantha Maragh, Paul B McCray, Todd C McDevitt, Oleg Mirochnitchenko, Ryuji Morizane, Niren Murthy, Randall S Prather, John A Ronald, Subhojit Roy, Sushmita Roy, Venkata Sabbisetti, Mark W Saltzman, Philip J Santangelo, David J Segal, Mary Shimoyama, Melissa C Skala, Alice F Tarantal, John C Tilton, George A Truskey, Moriel Vandsburger, Jonathan K Watts, Kevin D Wells, Scot A Wolfe, Qiaobing Xu, Wen Xue, Guohua Yi, and Jiangbing Zhou. 2021. “The NIH Somatic Cell Genome Editing program.” Nature, 592, 7853, Pp. 195-204. Abstract
Navin Gupta, Emre Dilmen, and Ryuji Morizane. 10/9/2020. “3D kidney organoids for bench-to-bedside translation.” Journal of Molecular Medicine. Abstract
Sandrine Ettou, Youngsook L. Jung, Tomoya Miyoshi, Dhawal Jain, Ken Hiratsuka, Valerie Schumacher, Mary E. Taglienti, Ryuji Morizane, Peter J. Park, and Jordan A. Kreidberg. 7/24/2020. “Epigenetic transcriptional reprogramming by WT1 mediates a repair response during podocyte injury.” Science Advances, 6, 30, Pp. eabb5460. Publisher's Version Abstract
Seiji Kishi, Craig R Brooks, Kensei Taguchi, Takaharu Ichimura, Yutaro Mori, Akinwande Akinfolarin, Navin Gupta, Pierre Galichon, Bertha C Elias, Tomohisa Suzuki, Qian Wang, Leslie Gewin, Ryuji Morizane, and Joseph V Bonventre. 11/1/2019. “Proximal tubule ATR regulates DNA repair to prevent maladaptive renal injury responses.” The Journal of Clinical Investigation, 11, 129, Pp. 4797-4816. Publisher's Version Abstract
Takuya Matsumoto, Olivier J.M. Schäffers, Wenqing Yin, and Ryuji Morizane. 7/30/2019. “Renal Regeneration: Stem Cell-Based Therapies to Battle Kidney Disease.” EMJ Nephrol., 7, 1, Pp. 54-64. Publisher's Version Abstract
Miyoshi T, Hiratsuka K, Garcia Saiz E, and Morizane R. 3/6/2019. “Kidney Organoids in Translational Medicine: Disease Modeling and Regenerative Medicine.” Developmental Dynamics. Publisher's Version Abstract
Morizane R. 2/18/2019. “Modelling diabetic vasculopathy with human vessel organoids.” Nature Reviews Nephrology. Publisher's Version Abstract
Kimberly A. Homan, Navin Gupta, Katharina T. Kroll, David B. Kolesky, Mark Skylar-Scott, Tomoya Miyoshi, Donald Mau, M. Todd Valerius, Thomas Ferrante, Joseph V. Bonventre, Jennifer A. Lewis, and Ryuji Morizane. 2/11/2019. “Flow-enhanced vascularization and maturation of kidney organoids in vitro .” Nature Methods. Publisher's Version Abstract
Morizane R. 1/31/2019. “Revealing potential cardiac manifestation of ADPKD using iPS cell-derived cardiomyocytes.” EBioMedicine. Publisher's Version
Hiratsuka K, Monkawa T, Akiyama T, Nakatake Y, Oda M, Goparaju SK, Kimura H, Chikazawa-Nohtomi N, Sato S, Ishiguro K, Yamaguchi S, Suzuki S, Morizane R, Ko SBH, Itoh H, and Ko MSH. 1/29/2019. “Induction of human pluripotent stem cells into kidney tissues by synthetic mRNAs encoding transcription factors.” Scientific Reports, 9, 1, Pp. 913. Abstract
Hill SJ, Decker B, Roberts EA, Horowitz NS, Muto MG, Worley MJ Jr, Feltmate CM, Nucci MR, Swisher EM, Nguyen H, Yang C, Morizane R, Kochupurakkal BS, Do KT, Konstantinopoulos PA, Liu JF, Bonventre JV, Matulonis UA, Shapiro GI, Berkowitz RS, Crum CP, and D'Andrea AD. 11/2018. “Prediction of DNA Repair Inhibitor Response in Short-Term Patient-Derived Ovarian Cancer Organoids.” Cancer Discov., 8, 11, Pp. 1404-1421. Abstract
Dario R Lemos, Michael McMurdo, Gamze Karaca, Julia Wilflingseder, Irina A Leaf, Navin Gupta, Tomoya Miyoshi, Koichiro Susa, Bryce G Johnson, Kirolous Soliman, Guanghai Wang, Ryuji Morizane, Joseph V Bonventre, and Jeremy S Duffield. 5/8/2018. “Interleukin-1β Activates a MYC-Dependent Metabolic Switch in Kidney Stromal Cells Necessary for Progressive Tubulointerstitial Fibrosis.” Journal of the American Society of Nephrology. Abstract
Navin Gupta, Koichiro Susa, Yoko Yoda, Joseph V. Bonventre, M. Todd Valerius, and Ryuji Morizane. 2018. “CRISPR/Cas9‐based Targeted Genome Editing for the Development of Monogenic Diseases Models with Human Pluripotent Stem Cells.” Current Protocols in Stem Cell Biology. Abstract
Ryuji Morizane and Joseph Bonventre. 2018. “Organoids for modeling kidney disease.” In Organs and Organoids, Pp. 227-245. Elsevier Inc. Abstract
The Division of Nephrology at Massachusetts General Hospital is a leading provider of services for patients with kidney disease, including diagnosis and management of kidney diseases and medical management of renal transplantation.
