Researchers from Mass General and colleagues have used an innovative organoid-on-a-chip model to identify new treatment targets for an inherited kidney disease that causes significant death and disability.
Morizane Lab: Ryuji Morizane, MD, PhD
Massachusetts General Hospital, Nephrology Division
149 13th Street
Boston, MA 02129
Explore This Research Lab
About the Morizane Lab
The Morizane Lab focuses on kidney disease studies using stem cell-derived tissues. Specifically, we are generating 3D kidney tissues termed organoids from human pluripotent stem cells (hPSCs). Kidney organoids consist of multiple cell types of kidneys, serving as a novel tool for drug development and regenerative medicine. Our group is divided into three main subgroups:
- Stem cell differentiation: We are creating novel methods to differentiate hPSCs into matured kidney tissues. We employ automated cell culture, organ-on-a-chip, 3D imaging and multiomics to improve the induced kidney tissues
- Kidney injury and repair: We are utilizing kidney organoids to understand intrinsic repair mechanisms of kidney epithelial cells. Our interests range from acute kidney injury to chronic kidney disease
- Genome editing and genetic kidney diseases: We are generating genetic disease models using genome editing and iPS reprogramming. Our current focus includes autosomal polycystic kidney disease
Ryuji Morizane, MD, PhD
Research Staff, Nephrology Division, Massachusetts General Hospital
Assistant Professor, Department of Medicine, Harvard Medical School
Affiliated Faculty, Harvard Stem Cell Institute
Visiting Scholar, Wyss Institute, Harvard University
Dr. Morizane is a physician-scientist at Massachusetts General Hospital and Harvard Medical School. He has a long-standing interest in translational research using hPSCs for kidney diseases and drug discovery. He has been working on pluripotent stem cell research since 2007 and established protocols to induce kidney lineage cells. His seminal work enabled the generation of nephron progenitor cells and kidney organoids from hPSCs with high efficiency, leading to his NIH Director’s New Innovator Award in 2019.
High school student
We accept fellows and visiting scientists from both academia and industry. Innovation Fellows Program enables personnel exchanges between our lab and participating industry. Should you have any questions, please contact Dr. Morizane.
Ryuji Morizane, MD, PhD
Navin Gupta, Takuya Matsumoto, Ken Hiratsuka, Edgar Garcia Saiz, Pierre Galichon, Tomoya Miyoshi, Koichiro Susa, Narihito Tatsumoto, Michifumi Yamashita, and Ryuji Morizane. 2022. “Modeling injury and repair in kidney organoids reveals that homologous recombination governs tubular intrinsic repair.” Sci Transl Med, 14, 634, Pp. eabj4772. Publisher's Version Abstract
Astia Rizki-Safitri, Tamara Traitteur, and Ryuji Morizane. 2021. “Bioengineered Kidney Models: Methods and Functional Assessments.” Function, 2, 4. Publisher's Version Abstract
Navin Gupta and Ryuji Morizane. 2021. “Kidney development to kidney organoids and back again.” Semin Cell Dev Biol. Abstract
Krishanu Saha, Erik J Sontheimer, PJ Brooks, Melinda R Dwinell, Charles A Gersbach, David R Liu, Stephen A Murray, Shengdar Q Tsai, Ross C Wilson, Daniel G Anderson, Aravind Asokan, Jillian F Banfield, Krystof S Bankiewicz, Gang Bao, Jeff WM Bulte, Nenad Bursac, Jarryd M Campbell, Daniel F Carlson, Elliot L Chaikof, Zheng-Yi Chen, Holland R Cheng, Karl J Clark, David T Curiel, James E Dahlman, Benjamin E Deverman, Mary E Dickinson, Jennifer A Doudna, Stephen C Ekker, Marina E Emborg, Guoping Feng, Benjamin S Freedman, David M Gamm, Guangping Gao, Ionita C Ghiran, Peter M Glazer, Shaoqin Gong, Jason D Heaney, Jon D Hennebold, John T Hinson, Anastasia Khvorova, Samira Kiani, William R Lagor, Kit S Lam, Kam W Leong, Jon E Levine, Jennifer A Lewis, Cathleen M Lutz, Danith H Ly, Samantha Maragh, Paul B McCray, Todd C McDevitt, Oleg Mirochnitchenko, Ryuji Morizane, Niren Murthy, Randall S Prather, John A Ronald, Subhojit Roy, Sushmita Roy, Venkata Sabbisetti, Mark W Saltzman, Philip J Santangelo, David J Segal, Mary Shimoyama, Melissa C Skala, Alice F Tarantal, John C Tilton, George A Truskey, Moriel Vandsburger, Jonathan K Watts, Kevin D Wells, Scot A Wolfe, Qiaobing Xu, Wen Xue, Guohua Yi, and Jiangbing Zhou. 2021. “The NIH Somatic Cell Genome Editing program.” Nature, 592, 7853, Pp. 195-204. Abstract
Navin Gupta, Emre Dilmen, and Ryuji Morizane. 10/9/2020. “3D kidney organoids for bench-to-bedside translation.” Journal of Molecular Medicine. Abstract
Sandrine Ettou, Youngsook L. Jung, Tomoya Miyoshi, Dhawal Jain, Ken Hiratsuka, Valerie Schumacher, Mary E. Taglienti, Ryuji Morizane, Peter J. Park, and Jordan A. Kreidberg. 7/24/2020. “Epigenetic transcriptional reprogramming by WT1 mediates a repair response during podocyte injury.” Science Advances, 6, 30, Pp. eabb5460. Publisher's Version Abstract
Seiji Kishi, Craig R Brooks, Kensei Taguchi, Takaharu Ichimura, Yutaro Mori, Akinwande Akinfolarin, Navin Gupta, Pierre Galichon, Bertha C Elias, Tomohisa Suzuki, Qian Wang, Leslie Gewin, Ryuji Morizane, and Joseph V Bonventre. 11/1/2019. “Proximal tubule ATR regulates DNA repair to prevent maladaptive renal injury responses.” The Journal of Clinical Investigation, 11, 129, Pp. 4797-4816. Publisher's Version Abstract
Takuya Matsumoto, Olivier J.M. Schäffers, Wenqing Yin, and Ryuji Morizane. 7/30/2019. “Renal Regeneration: Stem Cell-Based Therapies to Battle Kidney Disease.” EMJ Nephrol., 7, 1, Pp. 54-64. Publisher's Version Abstract
Miyoshi T, Hiratsuka K, Garcia Saiz E, and Morizane R. 3/6/2019. “Kidney Organoids in Translational Medicine: Disease Modeling and Regenerative Medicine.” Developmental Dynamics. Publisher's Version Abstract
Morizane R. 2/18/2019. “Modelling diabetic vasculopathy with human vessel organoids.” Nature Reviews Nephrology. Publisher's Version Abstract
Kimberly A. Homan, Navin Gupta, Katharina T. Kroll, David B. Kolesky, Mark Skylar-Scott, Tomoya Miyoshi, Donald Mau, M. Todd Valerius, Thomas Ferrante, Joseph V. Bonventre, Jennifer A. Lewis, and Ryuji Morizane. 2/11/2019. “Flow-enhanced vascularization and maturation of kidney organoids in vitro .” Nature Methods. Publisher's Version Abstract
Morizane R. 1/31/2019. “Revealing potential cardiac manifestation of ADPKD using iPS cell-derived cardiomyocytes.” EBioMedicine. Publisher's Version
Hiratsuka K, Monkawa T, Akiyama T, Nakatake Y, Oda M, Goparaju SK, Kimura H, Chikazawa-Nohtomi N, Sato S, Ishiguro K, Yamaguchi S, Suzuki S, Morizane R, Ko SBH, Itoh H, and Ko MSH. 1/29/2019. “Induction of human pluripotent stem cells into kidney tissues by synthetic mRNAs encoding transcription factors.” Scientific Reports, 9, 1, Pp. 913. Abstract
Hill SJ, Decker B, Roberts EA, Horowitz NS, Muto MG, Worley MJ Jr, Feltmate CM, Nucci MR, Swisher EM, Nguyen H, Yang C, Morizane R, Kochupurakkal BS, Do KT, Konstantinopoulos PA, Liu JF, Bonventre JV, Matulonis UA, Shapiro GI, Berkowitz RS, Crum CP, and D'Andrea AD. 11/2018. “Prediction of DNA Repair Inhibitor Response in Short-Term Patient-Derived Ovarian Cancer Organoids.” Cancer Discov., 8, 11, Pp. 1404-1421. Abstract
Dario R Lemos, Michael McMurdo, Gamze Karaca, Julia Wilflingseder, Irina A Leaf, Navin Gupta, Tomoya Miyoshi, Koichiro Susa, Bryce G Johnson, Kirolous Soliman, Guanghai Wang, Ryuji Morizane, Joseph V Bonventre, and Jeremy S Duffield. 5/8/2018. “Interleukin-1β Activates a MYC-Dependent Metabolic Switch in Kidney Stromal Cells Necessary for Progressive Tubulointerstitial Fibrosis.” Journal of the American Society of Nephrology. Abstract
Navin Gupta, Koichiro Susa, Yoko Yoda, Joseph V. Bonventre, M. Todd Valerius, and Ryuji Morizane. 2018. “CRISPR/Cas9‐based Targeted Genome Editing for the Development of Monogenic Diseases Models with Human Pluripotent Stem Cells.” Current Protocols in Stem Cell Biology. Abstract
Related News and Articles
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- Apr | 25 | 2022
The finding could have implications in the treatment of kidney diseases.
- Press Release
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Activating a DNA repair mechanism may help preserve kidney function in people with chronic kidney disease.
- Aug | 13 | 2021
In this interview, Kerry Reynolds, MD chats with Meghan Sise, MD, director of onconephrology at Mass General Hospital, to learn more about how immune checkpoint inhibitor (ICI) therapy affects kidney function.
- Apr | 7 | 2021
In this presentation from April 7, 2021, Winfred Williams, MD, and Jamil Sulemana, RN, BSN, discuss kidney disease in the black community, the differences between dialysis and kidney transplant, and when one should consider kidney transplantation.
- Patient Story
- Feb | 10 | 2021
When Ann Foti learned that she could become a living donor for her husband, Gino, she was determined to donate her kidney to him; however, because of complications with Gino’s condition and the onset of the COVID-19 pandemic, their journey toward transplantation surgery was far from simple.
About the Nephrology Division
The Division of Nephrology at Massachusetts General Hospital is a leading provider of services for patients with kidney disease, including diagnosis and management of kidney diseases and medical management of renal transplantation.