- Molybdenum cofactor (Moco) is a compound that is little known but is essential for life. Children born without the ability to synthesize Moco die young
- It has not been possible to create Moco supplements because the compound is so unstable.
- Studies in the nematode Caenorhabditis elegans suggest that by complexing, or combining, Moco with certain proteins, it becomes stable and can repair deficiency.
Kurt Warnhoff, PhD
We do not want to overstate our findings, especially as they relate to patients, but we are extremely excited about the therapeutic and fundamental implications of this work.
Department of Molecular Biology, Massachusetts General Hospital
BOSTON – Studies with a popular laboratory model, the nematode Caenorhabditis elegans, have revealed a possible therapeutic avenue for a rare but deadly condition in which children are born without the ability to make molybdenum cofactor (Moco) on their own.
Though little known, Moco is essential for life and so far it has proven extremely difficult to stably isolate this compound in the laboratory, preventing its use as a therapeutic supplement. This new work, which appears in Genes & Development, reveals that nematodes genetically engineered to be Moco-deficient can take up Moco that is attached to several types of proteins. This suggests that such protein-Moco complexes could be used as a treatment for Moco deficiency in people.
Children born with mutations that make them unable to synthesize Moco suffer lethal neurological and developmental defects. Providing them supplemental Moco might reverse these devastating symptoms. This work suggests a potential new route for delivering this essential cofactor.
The researchers carried out studies in C. elegans that were engineered to be deficient in their ability to make Moco. Like humans, C. elegans deficient in Moco die very early in development. Nematodes, however, can also ingest Moco from their diet – a process that is similar to supplementation, as with vitamins. The researchers found that the worms could take in Moco as a range of purified Moco-protein complexes. These included complexes with proteins from bacteria, bread mold, green algae and cow’s milk. Ingesting these complexes saved the Moco-deficient worms.
Further, they demonstrated that the Moco-protein complexes were very stable, suggesting it is possible to produce them as a supplement for children born with Moco deficiency.
“We tested four proteins and all four were restorative in our nematode model. That’s very encouraging,” says Kurt Warnhoff, PhD, an investigator in the Department of Molecular Biology at Massachusetts General Hospital (MGH) and the paper’s co-lead author. “We do not want to overstate our findings, especially as they relate to patients, but we are extremely excited about the therapeutic and fundamental implications of this work.”
The work was led by Gary Ruvkun, PhD, and Ralf R. Mendel, PhD. Ruvkun is at the Department of Molecular Biology at MGH and the Department of Genetics at Harvard Medical School. Mendel, a pioneer in Moco research, is at the Braunschweig University of Technology in Germany. Co-lead author Thomas W. Hercher is a member of Mendel’s lab.
The work was supported by the National Institutes of Health, the German Research Foundation and a Damon Runyon Fellowship.
About the Massachusetts General Hospital
Massachusetts General Hospital, founded in 1811, is the original and largest teaching hospital of Harvard Medical School. The Mass General Research Institute conducts the largest hospital-based research program in the nation, with annual research operations of more than $1 billion and comprises more than 9,500 researchers working across more than 30 institutes, centers and departments. In August 2020, Mass General was named #6 in the U.S. News & World Report list of "America’s Best Hospitals."