David N. Louis, MD, and Robert H. Young, MD, have received the 2020 Patricia R. Austen, RN, MGH History Award. The award honors individuals or groups who have worked to promote or preserve Mass General history.
Base editing by nucleotide deaminases linked to programmable DNA-binding proteins represents a promising approach to permanently remedy blood disorders, although its application in engrafting hematopoietic stem cells (HSCs) remains unexplored. In this study, we purified A3A (N57Q)-BE3 base editor for ribonucleoprotein (RNP) electroporation of human-peripheral-blood-mobilized CD34+ hematopoietic stem and progenitor cells (HSPCs). We observed frequent on-target cytosine base edits at the BCL11A erythroid enhancer at +58 with few indels. Fetal hemoglobin (HbF) induction in erythroid progeny after base editing or nuclease editing was similar. A single therapeutic base edit of the BCL11A enhancer prevented sickling and ameliorated globin chain imbalance in erythroid progeny from sickle cell disease and β-thalassemia patient-derived HSPCs, respectively. Moreover, efficient multiplex editing could be achieved with combined disruption of the BCL11A erythroid enhancer and correction of the HBB -28A>G promoter mutation. Finally, base edits could be produced in multilineage-repopulating self-renewing human HSCs with high frequency as assayed in primary and secondary recipient animals resulting in potent HbF induction in vivo. Together, these results demonstrate the potential of RNP base editing of human HSPCs as a feasible alternative to nuclease editing for HSC-targeted therapeutic genome modification.
- Aug | 25 | 2020
Phylogenetic analysis of SARS-CoV-2 in the Boston area highlights the role of recurrent importation and superspreading events.
SARS-CoV-2 has caused a severe, ongoing outbreak of COVID-19 in Massachusetts. To investigate the introduction, spread, and epidemiology of COVID-19 in the Boston area, we sequenced and analyzed 772 complete SARS-CoV-2 genomes from the region.
- Aug | 13 | 2020
Las pruebas y el rastreo generalizados son esenciales para controlar la propagación del COVID-19, pero hay varios tipos de pruebas diferentes, y cada una tiene un uso específico.
- Jun | 17 | 2020
Martha Pitman MD, director of the service, discusses the groundbreaking work taking place at the clinic and shares their new safety protocols