Bragg Lab — Chemical Neurobiology of Hereditary Dystonia
D. Cristopher Bragg, PhD
Director, Collaborative Center for XDP
149 13th Street
Charlestown, MA 02129
Explore This Laboratory
Research in the Bragg laboratory is directed towards the identification of pathogenic mechanisms underlying hereditary forms of dystonia and the development of screening tools to seek novel candidates for treating these disorders.
Their approach involves the derivation and characterization of dystonia cell culture models, including primary fibroblasts, lymphoblasts, and induced pluripotent stem cells (iPSCs), which can be used for probing cellular defects and testing candidate therapeutics.
Current projects are focused on studies of DYT1 and DYT6 dystonias, linked to mutations in TOR1Aand THAP1, respectively, as well as X-Linked Dystonia-Parkinsonism (XDP), for which the genetic lesion is not yet known.Visit the Collaborative Center for XDP
Induced pluripotent stem cell (iPSC) models of dystonia
Dystonia patient-specific iPSC lines are being generated by reprogramming of fibroblasts collected by the medical team at the Mass General Brigham Dystonia Clinic, directed by Dr. Nutan Sharma.
The goal of these studies is to differentiate patient and control iPSC lines into specific neuronal subtypes that have been implicated in the pathogenesis of dystonia. These differentiated neuronal cells then provide model systems for probing neuron-specific defects associated with particular dystonia genotypes.
Biochemistry of the DYT6 dystonia protein, THAP1
THAP1 is a zinc finger DNA binding protein involved in transcriptional regulation. More than 90 different mutations in THAP1 have been linked to dystonia in diverse patient populations throughout the world. Our group provided one of the first biochemical comparisons of different DYT6 mutant variants of THAP1, identifying a leucine zipper-type motif within its carboxy terminus that mediates self-oligomerization. Ongoing studies are characterizing the effects of dystonia mutations within different protein subdomains on (1) protein:protein interactions and (2) transcriptional activity.
Full PubMed publications list.
- Update on treatments for dystonia, 2014 Bragg DC, Sharma N Curr Neurol Neurosci Rep. 14(6):454.
- Dimerization of the DYT6 dystonia protein, THAP1, requires residues within the coiled-coil domain.2011 Sengel C, Gavarini S, Sharma N, Ozelius LJ, Bragg DC. J Neurochem. 118(6):1087-100.
- Molecular pathways in dystonia. 2010 Bragg DC, Armata IA, Nery FC, Breakefield XO, Sharma N. Neurobiol Dis. 2011 42(2):136-47
- Phosphorylation of the homer-binding domain of group I metabotropic glutamate receptors by cyclin-dependent kinase 5. 2009 Orlando LR, Ayala R, Kett LR, Curley AA, Duffner J, Bragg DC, Tsai LH, Dunah AW, Young AB. J Neurochem. 2009 110(2):557-69
- Dystonia-causing mutant torsinA inhibits cell adhesion and neurite extension through interference with cytoskeletal dynamics. 2006 Hewett JW, Zeng J, Niland BP, Bragg DC, Breakefield XO. Neurobiol Dis. 22(1):98-111.
- Impaired motor learning in mice expressing torsinA with the DYT1 dystonia mutation. Sharma N, Baxter MG, Petravicz J, Bragg DC, Schienda A, Standaert DG, Breakefield XO. 2005 J Neurosci. 25(22):5351-5.
- Inhibition of N-linked glycosylation prevents inclusion formation by the dystonia-related mutant form of torsinA. 2004 Bragg DC, Kaufman CA, Kock N, Breakefield XO. Mol Cell Neurosci. 2004 27(4):417-26.
- Perinuclear biogenesis of mutant torsin-A inclusions in cultured cells infected with tetracycline-regulated herpes simplex virus type 1 amplicon vectors. 2004 Bragg DC, Camp SM, Kaufman CA, Wilbur JD, Boston H, Schuback DE, Hanson PI, Sena-Esteves M, Breakefield XO. Neuroscience. 2004;125(3):651-61.
Our Research Team
Our team includes Christine Vaine, PhD, Instructor in Neurology; David Chin, Research Technician; and Kyle Chin, Research Technician.
Research at Mass General
Every day, our clinicians and scientists chart new terrain in biomedical research to treat and prevent human disease and advance patient care.